{"product_id":"abcam-ab277816","title":"Abcam, ab277816, Human GLI2 knockout A549 cell line","description":"\u003cp\u003eSize: 1000000Cells \/ vial\u003cbr\u003e\nGLI2 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Next Generation Sequencing,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAlthough we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.\u003cbr\u003e\nRecommended control\u003cbr\u003e\n: Human wild-type A549 cell line (ab288558). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-GLI2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGli2 also known as GLI family zinc finger 2 is a transcription factor with a molecular mass of approximately 160 kDa. It is an important component of the Hedgehog (Hh) signaling pathway. Gli2 primarily resides in the cytoplasm and migrates to the nucleus upon activation. Expression of Gli2 is widespread but most notable in developing tissues and adult brain. In the nucleus it regulates transcription by binding to specific DNA sequences.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGli2 acts as an activator or repressor of gene expression depending on the cellular context. It forms part of the Gli family of transcription factors playing a significant role in cellular growth differentiation and tissue patterning. While not usually part of stable protein complexes Gli2 interacts transiently with other proteins such as Suppressor of Fused (SuFu) to regulate its transcriptional activity. This interaction influences the processing and degradation of Gli proteins.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe Hedgehog signaling cascade prominently features Gli2 as a critical mediator. This pathway is fundamental in embryonic development. Alongside other Gli proteins like Gli1 and Gli3 Gli2 helps to translate extracellular Hh signals into precise cellular responses. In the absence of Hh ligands Gli2 remains tethered in the cytoplasm in a complex with SuFu limiting its transcription activity. Upon Hh pathway activation Gli2 translocates to the nucleus and modulates target gene expression.\u003cbr\u003e\nGli2 has a notable connection to cancers including basal cell carcinoma and medulloblastoma. Overactive Hedgehog signaling often involves dysregulation of Gli2 which promotes tumorigenesis by driving inappropriate cellular proliferation. Additionally Gli2 links to the genetic disorder Greig cephalopolysyndactyly syndrome via its interactions with Gli3 protein. These connections underline the importance of Gli2 as a therapeutic target in certain pathological conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845189095593,"sku":"ab277816","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab277816","provider":"Iright","version":"1.0","type":"link"}