{"product_id":"abcam-ab2786","title":"Abcam, ab2786, Anti-Endothelin 1 antibody [TR.ET.48.5]","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nAnti-Endothelin 1 antibody [TR.ET.48.5] (ab2786) is a mouse monoclonal antibody detecting Endothelin 1 in  Western Blot, Flow Cytometry, IHC-P, ICC\/IF . Suitable for  Human, Mouse, Rat . - Over 70 publications - Trusted since 2003\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:TR.ET.48.5,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:ICC\/IF, WB, Flow Cyt, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Native Full Length Protein corresponding to Human EDN1 conjugated to Keyhole Limpet Haemocyanin.P05305,\u003cbr\u003e\nEpitope:Studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16.,\u003cbr\u003e\nSpecificity:Immunohistochemical staining of ET-1 in human corpus cavernosum tissue with this antibody results in staining of endothelial cells.Radioimmune assays can be used to concentrate ET-1 in solution (e.g. serum\/plasma, milk, urine).\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-Endothelin 1 antibody [TR.ET.48.5] (ab2786) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of Endothelin 1?\u003cbr\u003e\nAnti-Endothelin 1 [TR.ET.48.5] (ab2786) specifically detects a band for Endothelin 1 (UniProt: P05305) at a molecular weight of 24kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-Endothelin 1 [TR.ET.48.5] (ab2786) was first used in a scientific publication in 2003 and has been cited over 70 times in peer-reviewed journals.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEndothelin 1 (ET-1) is a potent vasoconstrictor peptide with a molecular mass of approximately 2.5 kDa. The gene encoding ET-1 is expressed predominantly in endothelial cells where it exerts its vasoconstrictive effect. It is synthesized as a prepropeptide that undergoes cleavage to form the mature ET-1 peptide. The primary expression occurs in the vascular endothelial cells but it is also found in the kidney lung and brain indicating its widespread physiological role.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nET-1 regulates vascular tonus and blood pressure. It is part of the endothelin family that includes endothelin-2 (ET-2) and endothelin-3 (ET-3). ET-1 binds to endothelin receptors such as ETA and ETB which are G protein-coupled receptors facilitating cellular signaling processes. These receptors are abundant in smooth muscle cells and the central nervous system. The interaction of ET-1 with these receptors affects not only vascular smooth muscle contraction but also cell proliferation and production of extracellular matrix proteins.\u003cbr\u003e\nPathways\u003cbr\u003e\nET-1 plays an important role in the renin-angiotensin-aldosterone system and the nitric oxide signaling pathway. ET-1 interacts closely with angiotensin II a peptide hormone that increases blood pressure by stimulating the production of endothelin in endothelial cells. The release of nitric oxide by endothelium serves as a counter-regulatory mechanism to ET-1-mediated vasoconstriction. The balance between ET-1 and nitric oxide is vital for maintaining vascular homeostasis.\u003cbr\u003e\nET-1 is significantly implicated in hypertension and pulmonary arterial hypertension (PAH). The overproduction of ET-1 contributes to sustained high blood pressure and arterial stiffness. In PAH ET-1 levels are increased leading to the constriction and proliferation of pulmonary vascular cells. ET-1's interaction with proteins like transforming growth factor-beta (TGF-Β) further enhances its role in vascular remodeling associated with these conditions. Understanding ET-1's interaction in these pathways has made it a therapeutic target for treating these cardiovascular diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848521863337,"sku":"ab2786","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab2786","provider":"Iright","version":"1.0","type":"link"}