{"product_id":"abcam-ab279780","title":"Abcam, ab279780, Phosphotyrosine Eph receptor A3 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nPhosphotyrosine Eph receptor A3 ELISA Kit is a Semi-quantitative ELISA for the measurement of Phosphotyrosine Eph receptor A3 in Human in Cell\/Tissue Extracts samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Semi-quantitative,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPhospho-Eph receptor A3 ELISA Kit (ab279780) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Eph receptor A3 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.\u003cbr\u003e\nThis Sandwich ELISA kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the measurement of human phospho-Eph receptor A3. An anti-Eph receptor A3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated Eph receptor A3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-Eph receptor A3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEph receptor A3 also known as EphA3 is a member of the ephrin receptor subfamily of protein-tyrosine kinases. This receptor with a mass of approximately 120 kDa plays a role in cell-cell communication mainly by interacting with ephrin-A ligands. EphA3 is expressed in various tissues including the nervous system developing tissues and some adult tissues like the lungs and prostate. Its localization varies with the developmental stage and tissue type reflecting its role in numerous processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nEphA3 influences cellular organization movement and positioning through its involvement in contact-dependent signaling. It acts as part of a larger receptor-ligand complex which mediates bidirectional signaling between cells. This interaction generally leads to changes in the cytoskeleton impacting cell shape and locomotion. EphA3’s signaling can regulate developmental processes such as axon guidance angiogenesis and tissue boundary formation.\u003cbr\u003e\nPathways\u003cbr\u003e\nEphA3 modulates various important signaling pathways such as the PI3K\/AKT pathway and the Rho family GTPase pathway. It interacts with proteins like Ephrin-A5 and other Eph receptors coordinating complex cellular signaling networks. These interactions contribute to the regulation of cell proliferation survival and migration influencing tissue patterning and organ development.\u003cbr\u003e\nEphA3 has associations with cancer and neurological disorders. It has ties to various cancers like leukemia where it is implicated in proliferation and migration of malignant cells. In the context of neurological conditions EphA3 plays a role in neurodevelopmental disorders. In these diseases connections with other ephrin receptors and proteins involved in cell signaling become critical as they influence pathogenic pathways.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855660241065,"sku":"ab279780","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab279780","provider":"Iright","version":"1.0","type":"link"}