{"product_id":"abcam-ab279784","title":"Abcam, ab279784, Phosphotyrosine Eph receptor B3 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nPhosphotyrosine Eph receptor B3 ELISA Kit is a Semi-quantitative ELISA for the measurement of Phosphotyrosine Eph receptor B3 in Human in Cell\/Tissue Extracts samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Semi-quantitative,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPhospho-Eph receptor B3 ELISA Kit (ab279784) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Eph receptor B3 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.\u003cbr\u003e\nThis Sandwich ELISA kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the measurement of human and mouse phospho-Eph receptor B3. An anti-Eph receptor B3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated Eph receptor B3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-Eph receptor B3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEph receptor B3 also known as Ephrin type-B receptor 3 or EphB3 is a member of the Eph receptor family. These receptors are important elements in receptor tyrosine kinases notable for their involvement in numerous cellular processes. EphB3 has a molecular mass of approximately 110 kDa. EphB3 is predominantly expressed in the brain but also found in lungs intestines and spinal cord. Its expression pattern hints at its diverse roles in various organ systems.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nEph receptor B3 plays important roles in cellular communication influencing cell adhesion migration and differentiation. It often acts by forming receptor complexes with its ligands the ephrins. These interactions initiate bidirectional signaling which regulates the positioning of cells in developing tissues. EphB3 is important in guiding axon pathfinding in neurons and vascular patterning during development.\u003cbr\u003e\nPathways\u003cbr\u003e\nEph receptor B3 signaling influences the Rho family GTPase pathway. This pathway is involved in cytoskeletal dynamics and cellular morphology changes. EphB3 also partakes in the MAPK\/ERK pathway affecting cell proliferation and survival. Through the MAPK pathway EphB3 is linked with related proteins like EphA2 and EphB2 which share overlapping functions in cellular development and differentiation.\u003cbr\u003e\nEph receptor B3 has connections to cancer progression and neurological disorders. Changes in EphB3 expression levels have associations with colorectal cancer affecting cell invasion and metastasis. Additionally altered EphB3 signaling is reported in Alzheimer's disease where it seems to interact with the Amyloid precursor protein (APP) contributing to neurodegenerative processes. These associations highlight EphB3's importance in understanding and potentially targeting these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855654277289,"sku":"ab279784","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab279784","provider":"Iright","version":"1.0","type":"link"}