{"product_id":"abcam-ab280112","title":"Abcam, ab280112, Human HOXA9 knockout HEK-293 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nHOXA9 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 103 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 103 bp deletion in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-HOXA9, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHOXA9 also known as Homeobox A9 functions as a transcription factor and plays an important role in regulating gene expression during embryonic development and hematopoiesis. The protein has a mass of approximately 27 kDa. It shows expression in hematopoietic progenitor cells as well as in certain tissues like the lungs and the central nervous system. By binding to specific DNA sequences HOXA9 regulates genes that are vital for cellular differentiation and proliferation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nHOXA9 controls the transition and lineage commitment of hematopoietic stem cells. The protein often interacts as part of a transcriptional complex involving other HOX family proteins to maintain proper gene expression balance. This regulatory function is essential for normal gene expression patterns needed in stem cell differentiation and organ development processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nHOXA9 plays significant roles in the hematopoietic cell lineage and the retinoic acid signaling pathway. Through these pathways it collaborates with other HOX proteins like HOXB4 to maintain stem cell properties and influence the differentiation process. These interactions facilitate the production of necessary blood cell types and ensure effective response mechanisms to various developmental cues.\u003cbr\u003e\nHOXA9 shows a strong connection with acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS). Overexpression or mutation of HOXA9 disturbs normal hematopoietic processes often leading to these malignancies. The interaction with protein PBX1 also highlights its role in these diseases as their joint involvement frequently appears in leukemogenesis. Understanding how HOXA9 contributes to these pathways is critical for developing targeted therapeutic strategies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845213638825,"sku":"ab280112","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab280112","provider":"Iright","version":"1.0","type":"link"}