{"product_id":"abcam-ab281835","title":"Abcam, ab281835, Anti-TBX5 antibody [EPR23798-102] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal TBX5 antibody. Carrier free. Suitable for WB and reacts with Mouse samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR23798-102,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab281835 is the carrier-free version of\u003cbr\u003e\nab259980\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTBX5 also known as T-box transcription factor 5 is a protein that acts as a transcription factor. It has a mass of approximately 53 kDa. TBX5 is expressed significantly in the heart and limb tissues highlighting its importance in these areas. The protein contains a T-box domain that binds to specific DNA sequences allowing it to regulate the transcription of genes involved in developmental processes. TBX5 plays a critical role during embryogenesis particularly affecting cardiac and limb development.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe function of TBX5 extends to various cellular processes. It is involved in forming the cardiac septum and limb bud acting through its role as part of the transcriptional program. TBX5 doesn't typically form a complex with other proteins but interacts with partners like GATA4 to influence gene expression. These interactions enable TBX5 to activate or repress target genes necessary for organ and limb morphogenesis making it fundamental in developmental biology.\u003cbr\u003e\nPathways\u003cbr\u003e\nTBX5 operates within the cardiac development and limb differentiation pathways. It interacts with other critical factors such as NKX2-5 and TBX3 to coordinate the differentiation and proliferation processes essential for forming functional heart and limb structures. In heart development TBX5 and NKX2-5 synergistically work to activate cardiac-specific genes influencing the patterning and structural formation of the heart. The protein's involvement in limb differentiation emphasizes its dual role in these important developmental pathways.\u003cbr\u003e\nTBX5 has significant associations with conditions like Holt-Oram syndrome and congenital heart defects. Holt-Oram syndrome a disorder arising from mutations in the TBX5 gene is characterized by limb abnormalities and cardiac anomalies illustrating the protein's role in heart and limb development. TBX5 is also linked to cardiac arrhythmias with its molecular pathways involving proteins like NKX2-5. Mutations leading to TBX5 dysfunction impact these pathways resulting in developmental malformations and contributing to complex congenital conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46852526080169,"sku":"ab281835","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab281835","provider":"Iright","version":"1.0","type":"link"}