{"product_id":"abcam-ab282292","title":"Abcam, ab282292, Human Furin ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Furin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Furin with a sensitivity of 0.05 ng\/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Saliva, Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.05 ng\/mL,\u003cbr\u003e\nRange:0.16 - 10 ng\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Furin ELISA kit (ab282292) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Furin protein in human plasma, serum, saliva and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Furin with 0.05 ng\/mL sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n-Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n-High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n-Fully validated in biological samples\u003cbr\u003e\n-96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003cbr\u003e\nFurin, also referred to as dibasic-processing enzyme and Paired basic amino acid residue-cleaving enzyme (PACE), is a calcium-dependent serine endoprotease enzyme encoded by the FURIN gene. Furin belongs to the subtilisin-like proprotein convertase family S8. An 81 amino acid long inhibition peptide acts as a chaperone, is autocatalytically removed in the endoplasmic reticulum, and remains non-covalently bound until a second cleavage occurs in the trans Golgi. Furin acts on many substrates including but not limited to proparathroid hormone, transforming growth factor beta 1, proalbumin, and von Willebrand factor. Furin also acts on virus and is thought to activate the HIV envelope glycoproteins gp160 and gp140, and is thought to cleave the S1\/S2 site of the spike protein of the SARS-CoV-2 coronavirus.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nFurin is a serine endoprotease that plays critical role in the cleavage of precursor proteins into their active forms. It belongs to the proprotein convertase family and is also known as furin protease. The molecular weight of furin is approximately 54 kDa. Furin is ubiquitously expressed in various tissues in the body with high expression levels in the liver and the central nervous system. Furin is mainly located in the trans-Golgi network where it processes many precursor proteins involved in various cellular functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nFurin processes precursor proteins by cleaving at specific sequence motifs. This activity is essential for the maturation of many hormones growth factors and receptors. Furin is not part of a larger complex; it functions independently in its proteolytic activity. The processing by furin ensures that proteins become functional affecting numerous biological processes across different cell types and tissues.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe activity of furin impacts the regulation of the Notch signaling pathway and the MAPK\/ERK pathway. In the Notch signaling pathway furin processes Notch receptors facilitating their activation and downstream signaling. In the MAPK\/ERK pathway furin activates several key proteins including growth factors and receptors essential for cell proliferation and differentiation. Furin-related proteins such as convertase enzymes are part of a similar family having roles in overlapping or complementary pathways.\u003cbr\u003e\nFurin is implicated in several conditions including cardiovascular diseases and certain cancers. In cardiovascular diseases furin's role in processing proteins such as pro-BNP may have implications in cardiac function and disease progression. In cancer altered furin activity can lead to the inappropriate activation of growth factors and receptors contributing to tumor progression. Furin inhibitors are researched for their potential to modulate furin activity in these diseases and provide therapeutic benefits.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843591360681,"sku":"ab282292","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab282292","provider":"Iright","version":"1.0","type":"link"}