{"product_id":"abcam-ab282296","title":"Abcam, ab282296, EIF2S1 ELISA Kit (pS51)","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nEIF2S1 ELISA Kit (pS51) is a single-wash 90-min Simplestep used to quantify EIF2S1 (pS51) with a sensitivity of 9 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 9 pg\/mL,\u003cbr\u003e\nRange:51.56 - 3300 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nEIF2S1 ELISA Kit (pS51) ab282296 is a rapid single-wash 90-min Sandwich ELISA to measure EIF2S1 (pS51)in cell lysate. This SimpleStep sensitivity is 9 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nEIF2S1 (pS51)SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nEIF2S1 ELISA Kit (pS51) ab282296 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nEIF2S1, Eukaryotic translation initiation factor 2 subunit 1, plays a key role in the initiation of protein synthesis. Dysregulation of EIF2S1 plays a part in the development of type II diabetes and ischemic injury. The immunogen from which the antibodies in this kit were raised represents the full-length mature protein. This protein shares homology across Mammalia, and therefore is expected to cross-react with many species. The protein standard in this product is recombinant human EIF2S1 ligated with peptide containing phosphorylated serine 51.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEIF2S1 also called eIF2? is a subunit of the eukaryotic translation initiation factor 2 (eIF2) complex. This protein plays an important role in the initiation of protein synthesis by regulating the binding of the initiator methionyl-tRNA to the 40S ribosomal subunit. Its molecular weight is approximately 36 kDa. EIF2S1 is widely expressed in various tissues providing a fundamental function in protein biosynthesis across different cell types.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nEIF2? acts as a regulatory component of the eIF2 complex which is central to initiating translation in eukaryotic cells. Phosphorylation of eIF2? leads to inhibition of the exchange of GDP to GTP on eIF2 effectively reducing global protein synthesis while allowing the translation of specific mRNAs involved in stress responses. The eIF2? forms part of the eIF2 complex working closely with other subunits to exert its regulatory functions.\u003cbr\u003e\nPathways\u003cbr\u003e\nEIF2? is involved in the integrated stress response (ISR) and other protein synthesis pathways. The phosphorylation state of eIF2? is important in ISR linking it to proteins such as PERK and GCN2 which respond to various stress signals. Phospho-eIF2? plays a significant role in modulating the translation response to cellular stress ensuring cells can adapt or initiate apoptosis depending on the conditions.\u003cbr\u003e\nEIF2? connects to diseases like Alzheimer's and diabetes. Alterations in phosphorylation of eIF2? are linked to endoplasmic reticulum stress in Alzheimer's affecting protein homeostasis. Connecting to diabetes dysregulated eIF2? signaling interferes with insulin synthesis and secretion. Proteins like ATF4 also intersect with eIF2? in these diseases highlighting eIF2?'s involvement in critical pathological processes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843585659049,"sku":"ab282296","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab282296","provider":"Iright","version":"1.0","type":"link"}