{"product_id":"abcam-ab282297","title":"Abcam, ab282297, Mouse Prealbumin ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests \/ 1 x 384Tests \/ 10 x 96Tests\u003cbr\u003e\nMouse Prealbumin ELISA Kit is a single-wash 90-min SimpleStep Sandwich ELISA used to quantify Mouse Prealbumin (Transthyretin, TTR) with a sensitivity of 62.48 pg\/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric sandwich ELISA - 450 nm readout, works on any plate reader - Different formats for different needs: 384-well for higher throughput - Need to design your own assay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 62.48 pg\/mL,\u003cbr\u003e\nRange:234.38 - 15000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse Prealbumin ELISA Kit ab282297 is a rapid single-wash 90-min ELISA kit to measure Mouse Prealbumin in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma. This SimpleStep ELISA® sensitivity is 5.64 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nMouse Prealbumin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nThis kit is designed for the quantification of mouse Prealbumin. The standard protein in this kit is full length mouse Prealbumin. Native signal was detected in serum, plasma (citrate), plasma (EDTA), plasma (heparin), and tissue culture supernatant sample types. Cell extract, tissue extract, urine, saliva, CSF, and milk samples have not been tested with this kit.\u003cbr\u003e\nMouse Prealbumin ELISA Kit ab282297 protocol summary:\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450nm\u003cbr\u003e\nSave your precious samples\u003cbr\u003e\nOur 384-well Mouse Prealbumin ELISA Kit only requires a maxmum of 12.5µL\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation\u003cbr\u003e\n- Mouse Prealbumin Antibody Pair - BSA and Azide free\u003cbr\u003e\nab253737\u003cbr\u003e\n- Anti-Prealbumin antibody [EPR20971-130] - BSA and Azide free (Capture)\u003cbr\u003e\nab283848\u003cbr\u003e\n- Anti-Prealbumin antibody [EPR20971-140] - BSA and Azide free (Detector)\u003cbr\u003e\nab283849\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nMouse Prealbumin ELISA Kit ab282297 is commonly used to study nutrition and endocrine disorders.\u003cbr\u003e\nTo measure prealbumin in human samples:\u003cbr\u003e\n- Human Prealbumin ELISA kit\u003cbr\u003e\nab231920\u003cbr\u003e\nPrealbumin (Transthyretin, or TTR) is a transport protein for thyroxine and retinol-binding protein bound to retinol. Prealbumin forms a dimer of dimers, and misfolding of it is associated with various amyloid diseases. Prealbumin levels are an indication of protein-calorie nutrition and has been used to monitor the effectiveness of parenteral nutrition.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843569340585,"sku":"ab282297","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab282297","provider":"Iright","version":"1.0","type":"link"}