{"product_id":"abcam-ab282299","title":"Abcam, ab282299, Human S100A12\/CGRP ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman S100A12\/CGRP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human S100A12\/CGRP with a sensitivity of 1.89 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 1.89 pg\/mL,\u003cbr\u003e\nRange:7.81 - 500 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman S100A12\/CGRP ELISA Kit ab282299 is a rapid single-wash 90-min Sandwich ELISA to measure Human S100A12\/CGRP in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 1.89 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman S100A12\/CGRP SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nHuman S100A12\/CGRP ELISA Kit ab282299 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:\u003cbr\u003e\n- Anti-S100A12\/CGRP antibody [EPR23677-121] - BSA and Azide free (Capture)\u003cbr\u003e\nab281062\u003cbr\u003e\n- Anti-S100A12\/CGRP antibody [EPR23677-102] - BSA and Azide free (Detector)\u003cbr\u003e\nab281062\u003cbr\u003e\nS100A12, also known as CCRP and calgranulin C, is encoded by the\u003cbr\u003e\nS100A12\u003cbr\u003e\ngene and a member of the S100 family. Found secreted by neutrophils, monocytes, and keratinocytes in which the protein plays significant roles as antiparasitic, anti-infectious, and antibacterial. Moreover, oligomerization and target interactions by S100A12 is regulated by both zinc and calcium ions resulting in cell signaling and changes in cellular microenvironment.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe S100A12 protein also known as Calgranulin C or CGRP (Calcitonin Gene-Related Peptide) has a molecular mass of approximately 10.4 kDa. This protein shows expression primarily in neutrophils but is also present in other types of cells such as epithelial cells and certain cell lines. S100A12 belongs to the S100 protein family which is part of the EF-hand calcium-binding proteins known for playing roles in cell regulation. The protein binds calcium ions which influence its structural conformation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nS100A12 is important in the inflammatory response and immune system regulation. It acts as a pro-inflammatory mediator attracting leukocytes to sites of inflammation. Furthermore it can bind to receptors on cell surfaces like RAGE (Receptor for Advanced Glycation End Products) which induces signaling cascades that amplify immune responses. S100A12 does not function alone but often forms homodimers or heterodimers with other S100 proteins enhancing its biological activities.\u003cbr\u003e\nPathways\u003cbr\u003e\nS100A12 participates in pathways like the inflammatory response pathway and the RAGE signaling pathway. By interacting with RAGE and possibly other receptors it impacts cellular responses associated with stress injury and microbial infection. This interaction connects S100A12 to other proteins in the same pathways such as NF-kB an important transcription factor involved in inflammation and immune responses.\u003cbr\u003e\nS100A12 has connections with conditions like rheumatoid arthritis and inflammatory bowel disease. Elevated levels of S100A12 often correlate with disease activity and severity suggesting its potential as a biomarker. In rheumatoid arthritis it contributes to the inflammatory environment while in inflammatory bowel disease it associates not only with inflammation but also with proteins like IL-6 and TNF-alpha which are significant cytokines in the pathogenesis of these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843560919209,"sku":"ab282299","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab282299","provider":"Iright","version":"1.0","type":"link"}