{"product_id":"abcam-ab282875","title":"Abcam, ab282875, Rat IL-1Ra ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nRat IL-1Ra ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat IL-1Ra with a sensitivity of 6.95 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture supernatant,\u003cbr\u003e\nReacts with:Rat,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 6.95 pg\/mL,\u003cbr\u003e\nRange:23.44 - 1500 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nRat IL-1Ra ELISA kit (ab282875) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Rat IL-1Ra protein in rat cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-1Ra with 6.95 pg\/mL sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n-Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n-High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n-Fully validated in biological samples\u003cbr\u003e\n-96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003cbr\u003e\nInterleukin-1 receptor antagonist (IL-1Ra), encoded by the\u003cbr\u003e\ngene, is a cell surface receptor and a member of the interleukin-1 cytokine family. IL-1Ra inhibits the activity of IL-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. However, IL-1Ra has no IL-1 like activity. Studies have shown the absence of IL-1Ra allows unopposed action of IL-1, resulting in life-threating systemic inflammation with skin and bone involvement thus suggesting IL-1Ra to play a role in modulating pro-inflammatory responses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843538047145,"sku":"ab282875","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab282875","provider":"Iright","version":"1.0","type":"link"}