{"product_id":"abcam-ab282881","title":"Abcam, ab282881, Human DR4 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman DR4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human DR4 with a sensitivity of 1.92 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 1.92 pg\/mL,\u003cbr\u003e\nRange:4.688 - 300 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman DR4 ELISA kit (ab282881) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human DR4 protein in human cell extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DR4 with 5 pg\/mL sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n-Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n-High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n-Fully validated in biological samples\u003cbr\u003e\n-96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003cbr\u003e\nHuman DR4 is a type 1, TNF R family, membrane protein. Human DR4 is a receptor for the cytotoxic ligand TNFSF10\/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Human DR4 also promotes the activation of NF-kappa-B. DR4 is most closely related to DR5 sharing a 55% amino acid sequence identity.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nDR4 also known as TRAIL-R1 is a receptor with a molecular weight of approximately 60 kDa. It is part of the tumor necrosis factor receptor (TNFR) superfamily. DR4 is widely expressed in various types of tissues such as the liver spleen thymus and peripheral blood leukocytes. It functions mechanically by binding to its ligand TNF-related apoptosis-inducing ligand (TRAIL) which initiates signaling cascades that lead to apoptosis or programmed cell death.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe death receptor 4 plays an important role in mediating cell apoptosis. Upon ligand binding DR4 interacts with the adapter molecule FADD (FAS-associated protein with death domain) facilitating the formation of the death-inducing signaling complex (DISC). This complex recruits and activates caspase-8 which eventually leads to the activation of the downstream effector caspases driving the cell towards apoptosis. DR4 operates as a significant component of the extrinsic pathway of apoptosis a process important for maintaining cellular homeostasis and eliminating harmful cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nDR4 functions critically within the extrinsic apoptotic pathway. This pathway involves other death receptors such as DR5 which also binds TRAIL similarly leading to apoptosis through DISC formation and caspase activation. The extrinsic pathway interconnects with the intrinsic pathway via the mitochondria with proteins like Bid acting as a bridge between them. The engagement of these pathways highlights DR4’s importance in regulatory mechanisms of cell death tightly controlling cellular proliferation and survival.\u003cbr\u003e\nDR4 plays a significant role in cancer and autoimmune diseases. Alterations in DR4 expression or function can lead to evasion of apoptosis by cancer cells contributing to tumor progression. The defective apoptotic signaling via DR4 is also linked to autoimmune disorders where insufficient apoptosis may allow for the survival of autoreactive immune cells. In both scenarios DR4's involvement usually associates with its counterpart DR5 as they share similar ligand-binding properties and signal transduction mechanisms in diseased states.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843581268137,"sku":"ab282881","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab282881","provider":"Iright","version":"1.0","type":"link"}