{"product_id":"abcam-ab283588","title":"Abcam, ab283588, Anti-KCC2 antibody [EPR24203-85] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal KCC2 antibody. Carrier free. Suitable for ICC\/IF, IP, WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR24203-85,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:WB, IP, IHC-Fr, Flow Cyt (Intra), IHC-P, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab283588 is the carrier-free version of\u003cbr\u003e\nab259969\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nKCC2 also known as the potassium-chloride cotransporter 2 or SLC12A5 is an important neuronal protein. This transporter weighs approximately 125 kDa and is predominantly found in the central nervous system especially in neurons. KCC2 plays a central role in maintaining the intracellular chloride ion concentration by extruding chloride ions out in exchange for potassium ions. Through this action it aids in establishing the inhibitory action of neurotransmitters like GABA and glycine.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nKCC2 contributes significantly to the regulation of neuronal excitability and synaptic transmission. It does not appear as part of a larger protein complex but functions as a homodimer. KCC2's activity helps determine the strength and efficacy of inhibitory synapses influencing processes such as synaptic plasticity and neural development. By managing chloride ion gradients KCC2 ensures proper inhibitory neurotransmission which is essential for balanced neural activity.\u003cbr\u003e\nPathways\u003cbr\u003e\nKCC2 is important for the GABAergic neurotransmission pathway which is significant for inhibitory signaling in the nervous system. This transporter interacts closely with proteins like NKCC1 another cation-chloride cotransporter which has an opposing function in chloride ion movement. The GABAergic pathway relies on KCC2 for hyperpolarizing inhibition contributing to the regulation of neuronal networks and stabilizing membrane potential.\u003cbr\u003e\nKCC2 malfunctions associate with epilepsy and neuropathic pain. Altered expression or dysfunction of KCC2 disrupts inhibitory control leading to abnormal hyperexcitability in neurons which is a characteristic of epilepsy. Moreover in neuropathic pain downregulation of KCC2 impairs glycinergic inhibition contributing to the pathophysiology of chronic pain states. In these conditions KCC2's interaction with proteins such as NKCC1 highlights its role in maintaining inhibitory balance and neural homeostasis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851606577321,"sku":"ab283588","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab283588","provider":"Iright","version":"1.0","type":"link"}