{"product_id":"abcam-ab284052","title":"Abcam, ab284052, Anti-SARS-CoV-2 nsp16 antibody [EPR24841-18] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal R1AB antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC\/IF, WB, I-ELISA and reacts with Transfected cell line - SARS-CoV-2, Recombinant full length protein - SARS-CoV-2 samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR24841-18,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:SARS-CoV-2,\u003cbr\u003e\nApplications:I-ELISA, ICC\/IF, Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab284052 is the carrier-free version of\u003cbr\u003e\nab284038\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSARS-CoV-2 nsp16 also known as non-structural protein 16 is an important enzyme in the replication and transcription machinery of the virus. It exhibits 2'-O-methyltransferase activity and has a molecular mass of approximately 33 kDa. Nsp16 is expressed in host cells once the virus infects the cell and translates its polyproteins. Its main role is to modify the cap structure of viral mRNA which helps in avoiding host immune detection and promoting efficient viral replication.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe function of nsp16 centers around its interaction with nsp10 forming a stable 2'-O-methyltransferase complex. This enzyme complex is responsible for the methylation of the ribonucleic acid cap at the first nucleotide. This cap modification is necessary for the stability of the viral mRNA and its ability to be recognized by the host's cellular machinery as native mRNA which prevents antiviral defenses from targeting the virus. The methylation activity of nsp16 is highly specific requiring both nsp10 and nsp16 to function together effectively.\u003cbr\u003e\nPathways\u003cbr\u003e\nNsp16 plays an integral role in the viral replication-transcription complex (RTC) pathway. This pathway is essential for the synthesis of viral genomic and subgenomic RNAs facilitating the life cycle of SARS-CoV-2. The RTC includes other viral proteins like nsp12 which acts as the RNA-dependent RNA polymerase. These interactions ensure the replication fidelity and quantity necessary for viral protein production and spreading within the host organism.\u003cbr\u003e\nNsp16 is connected to COVID-19 the disease caused by SARS-CoV-2 infection. Its role in immune evasion makes it a target for antiviral drug development as disabling the nsp16 function could make the virus more susceptible to host immune responses. nsp16's cooperation with nsp10 is also significant in this context as disrupting their interaction might prevent the virus from effectively replicating offering a potential therapeutic strategy to combat COVID-19.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851262513321,"sku":"ab284052","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab284052","provider":"Iright","version":"1.0","type":"link"}