{"product_id":"abcam-ab284619","title":"Abcam, ab284619, Rat Kallikrein 10 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nRat Kallikrein 10 ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Kallikrein 10 with a sensitivity of 2.35 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture media, Heparin Plasma, Serum,\u003cbr\u003e\nReacts with:Rat,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 2.35 pg\/mL,\u003cbr\u003e\nRange:10.16 - 650 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nRat Kallikrein 10 ELISA kit (\u003cbr\u003e\nab272199\u003cbr\u003e\n) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Rat Kallikrein 10 protein in rat serum, plasma, and cell culture media samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Kallikrein 10 with 2.35 pg\/mL sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n-Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n-High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n-Fully validated in biological samples\u003cbr\u003e\n-96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nKallikrein 10 also known as KLK10 or serine protease 20 is a protein known for its enzymatic activity. It has a mass of approximately 30 kDa and expresses highly in tissues like the pancreas kidney and hormonal dependent tissues such as the prostate and breast. KLK10 involves in the degradation of extracellular matrix components functioning as a serine protease. Its expression in hormone-responsive tissues indicates a potential role in processes like tissue remodeling and regulation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nKallikrein 10 is part of a larger family of serine proteases involved in various physiological processes. These proteases participate in tissue homeostasis and in some instances immune response. KLK10 expression links to cell proliferation and survival potentially influencing cancer progression. The network of serine proteases forms a proteolytic cascade where multiple proteases sequentially activate amplifying the biological responses.\u003cbr\u003e\nPathways\u003cbr\u003e\nKallikrein 10 plays a critical role in the proteolytic cascade responsible for regulating homeostatic processes. It participates notably in pathways such as the kallikrein-kinin system and the regulation of tumor microenvironment. KLK10 interacts with other kallikreins like KLK5 and KLK6 mediating their activation and functioning in tissue remodeling and repair processes.\u003cbr\u003e\nKallikrein 10 has a significant association with cancers and inflammatory conditions. Altered KLK10 expression shows correlation with prostate cancer and breast cancer where it might serve as a biomarker for diagnosis or prognosis. KLK10's involvement with proteins like KLK3 (PSA) in prostate cancer reflects its potential utility in therapeutic strategies especially concerning hormone-dependent tumors.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843588280489,"sku":"ab284619","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab284619","provider":"Iright","version":"1.0","type":"link"}