{"product_id":"abcam-ab284625","title":"Abcam, ab284625, Human REST \/ NRSF ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman REST \/ NRSF ELISA Kit is a single-wash 90-min Simplestep used to quantify Human REST \/ NRSF with a sensitivity of 11.6 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 11.6 pg\/mL,\u003cbr\u003e\nRange:39 - 2500 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman REST \/ NRSF ELISA kit (ab284625) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human REST \/ NRSF protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human REST \/ NRSF with 11.6 pg\/mL sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n-Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n-High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n-Fully validated in biological samples\u003cbr\u003e\n-96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nREST also known as NRSF (neuron-restrictive silencer factor) is a transcriptional repressor weighing approximately 116 kDa. It regulates gene expression by binding to the RE1 silencing transcription factor (RE1-silencing element) in the DNA. REST is widely expressed in non-neuronal cells and plays a significant role in maintaining neuronal gene silencing in embryonic and adult stem cells. By interacting with co-repressors REST recruits histone deacetylases and methylases to modify chromatin structure effectively shutting down gene transcription in specific contexts.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nREST regulates neuronal differentiation and plasticity by controlling the expression of neuronal-specific genes. It forms a complex with co-repressors such as mSin3 and CoREST participating in diverse biological processes including neurogenesis. REST expression is important for balancing the neuronal gene expression program preventing untimely or inappropriate activation of neuronal genes in non-neuronal tissues. By modulating the expression of synaptic proteins and neurotransmitter receptors REST influences synaptic plasticity and neuronal excitability.\u003cbr\u003e\nPathways\u003cbr\u003e\nREST participates in the neural stem cell differentiation pathway and cell fate determination. It interacts with pathways involving proteins such as HDAC1 and SWI\/SNF complexes contributing to chromatin remodeling and transcriptional regulation. Within these pathways REST ensures the maintenance of non-neuronal identity by repressing neuronal gene activation coordinating with other proteins to influence cellular outcomes and fate decisions.\u003cbr\u003e\nREST has been linked to neurological disorders such as Huntington's disease and epilepsy. In Huntington's disease REST dissociation from the Huntingtin protein increases leading to misregulation of neuronal genes and contributing to neurodegenerative changes. REST also plays a role in epilepsy where its dysregulation can lead to an imbalance in excitatory and inhibitory signals within the brain potentially triggering epileptic episodes. Through its interactions with disease-associated proteins REST exemplifies the complex interplay between transcriptional regulation and cellular dysfunction in disease pathology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843588378793,"sku":"ab284625","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab284625","provider":"Iright","version":"1.0","type":"link"}