{"product_id":"abcam-ab285065","title":"Abcam, ab285065, Anti-FAP antibody [EPR24291-6] - BSA and Azide free (Detector)","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal Fibroblast activation protein, alpha antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR24291-6,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nApplications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab285065 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse FAP. The recommended pair for sandwich ELISA is:\u003cbr\u003e\nCapture:\u003cbr\u003e\nab284959\u003cbr\u003e\n, Mouse FAP Capture Antibody (unconjugated)\u003cbr\u003e\nDetector: ab285065, Mouse FAP Detector Antibody (unconjugated)\u003cbr\u003e\nThe reference range value is 31 - 2000 pg\/mL.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nSandwich ELISA\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nFAP also known as fibroblast activation protein alpha or seprase is a serine protease with a mass of approximately 97 kDa. It is largely expressed in embryonic tissues certain carcinomas and tissue remodeling sites. FAP acts mechanistically by exhibiting dipeptidyl peptidase and endopeptidase activities breaking down peptide chains. Cellular membrane-bound FAP localizes mainly on reactive stromal fibroblasts not normal tissues making it an interesting target for research.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nFAP plays a role in tissue remodeling and wound healing influencing the microenvironment of tumors and fibrotic tissues. It contributes to extracellular matrix degradation participating in the complex network of proteases. FAP is not a standalone unit but part of a larger complex that interacts with other proteases helping in cell migration and invasion. As a result its activity impacts processes like inflammation and carcinogenesis.\u003cbr\u003e\nPathways\u003cbr\u003e\nFAP participates in processes related to tissue repair and cancer progression. Significant pathways include the degradation of the extracellular matrix and the PI3K\/AKT signaling. It functions alongside proteins like DPP4 another dipeptidyl peptidase in these pathways. The matrix breakdown is central to cancer metastasis and wound healing enhancing our understanding of FAP's role in larger biological contexts.\u003cbr\u003e\nFAP has links with cancer and fibrosis. Its expression correlates with aggressive tumors and poor prognosis in cancers such as breast and ovarian. In fibrotic diseases like pulmonary and liver fibrosis it contributes to excessive matrix deposition and tissue stiffening. FAP's involvement alongside collagenases in these disorders makes it a target of potential therapeutic interest.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46852284350633,"sku":"ab285065","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab285065","provider":"Iright","version":"1.0","type":"link"}