{"product_id":"abcam-ab285242","title":"Abcam, ab285242, Mouse Total cholesterol ELISA Kit","description":"\u003cp\u003eSize: 96Test\u003cbr\u003e\nMouse Total cholesterol ELISA Kit is a sandwich ELISA designed to quantify Mouse Total cholesterol - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 78 - 5000 pg\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (qualitative),\u003cbr\u003e\nRange:78 - 5000 pg\/mL,\u003cbr\u003e\nAssay time:90m,\u003cbr\u003e\nAssay Platform:Microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse Total cholesterol ELISA Kit ab285242 is a sandwich ELISA to measure Mouse Total cholesterol in serum, plasma, cell culture supernatant, tissue homogenate, other biological fluids.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nThe entire process can be performed within 90 minutes with only a single incubation and a wash step respectively, resulting in fewer handling steps, fewer errors and more consistent results.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nMouse Total cholesterol ELISA Kit ab285242 protocol summary\u003cbr\u003e\n1. Add standards or samples to appropriate wells. Incubate\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add HRP conjugate reagent to all wells. Incubate\u003cbr\u003e\n4. Wash\u003cbr\u003e\n5. Add Chromogen solutions to each well. Incubate.\u003cbr\u003e\n6. Add stop solution and read at 450 nm.\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K4440 QuickDetect™ Total cholesterol (Mouse) ELISA Kit. K4440-100 is the same size as the 96 test size of ab285242.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCholesterol sometimes referred to as fchol is a lipid molecule with a molecular mass of approximately 386.65 Daltons. It is an essential component of cell membranes and is found in the plasma membranes of cells throughout the body. Cholesterol primarily resides in the membranes where it modulates fluidity and mechanical properties. Its expression occurs in various tissues but it is abundant in the liver which plays an important role in its synthesis and regulation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCholesterol is important in maintaining the structural integrity and organization of membranes. It is also part of lipid rafts which are specialized microdomains within the cell membrane that facilitate cellular signaling. Cholesterol interacts with proteins and receptors embedded in the membrane influencing their function. It does not form a complex itself but affects complexes within the membrane like lipid rafts and signaling platforms.\u003cbr\u003e\nPathways\u003cbr\u003e\nCholesterol is a critical component of the steroid biosynthesis pathway and the bile acid synthesis pathway. It serves as a precursor for steroid hormones including cortisol and sex hormones as well as bile acids which are essential for digestion and lipid absorption. Proteins such as HMG-CoA reductase and the low-density lipoprotein receptor (LDLR) are key players related to cholesterol in these pathways regulating its synthesis and uptake in cells.\u003cbr\u003e\nCholesterol is closely associated with atherosclerosis and cardiovascular disease. Elevated levels of low-density lipoprotein (LDL) cholesterol contribute to plaque formation in arteries increasing the risk of heart attack and stroke. On the other hand high-density lipoprotein (HDL) cholesterol helps remove excess cholesterol from tissues including the arterial walls. Statins a class of drugs that lower LDL cholesterol levels target HMG-CoA reductase and are commonly used in treating hypercholesterolemia and cardiovascular diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843631698089,"sku":"ab285242","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab285242","provider":"Iright","version":"1.0","type":"link"}