{"product_id":"abcam-ab285264","title":"Abcam, ab285264, Rat Alanine Aminotransferase (ALT) ELISA Kit","description":"\u003cp\u003eSize: 96Test\u003cbr\u003e\nRat Alanine Aminotransferase (ALT) ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat Alanine Aminotransferase (ALT) in Rat in Biofluids, Cell\/Tissue Extracts samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Tissue Homogenate, Serum, Other biological fluids,\u003cbr\u003e\nReacts with:Rat,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 0.469 ng\/mL,\u003cbr\u003e\nRange:0.78 - 50 ng\/mL,\u003cbr\u003e\nAssay Platform:Microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAlanine aminotransferase 1 mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.\u003cbr\u003e\nThis GPT ELISA kit (ab285264, E4325) is a sandwich ELISA assay for the quantitative measurement of rat GPT in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of rat GPT captured from the samples\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called E4325 Alanine Aminotransferase 1 (GPT) (rat) ELISA Kit. E4325-100 is the same size as the 96 test size of ab285264.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAlanine transaminase also known as ALT or alanine aminotransferase is an important enzyme in the body. It has a molecular mass of approximately 54 kDa and catalyzes the conversion between alanine and pyruvate by transferring an amino group. ALT is mainly expressed in the liver but also present in other tissues like the heart and muscles. It is often measured as 'ALT activity' in clinical settings to ascertain liver health. In laboratories an 'ALT assay' or 'ALT kit' can determine alanine transaminase activity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAlanine transaminase plays a critical role in amino acid metabolism and energy production. It facilitates the transamination process which is significant for the balance of amino acids and carbohydrates. ALT as a standalone enzyme does not form part of a protein complex but works closely with alanine aminotransferase reactions. It helps maintain the cellular nitrogen balance linking nitrogen metabolism with energy pathways.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe role of alanine transaminase is to interconnect carbohydrate and amino acid metabolism. It participates in the gluconeogenesis pathway and the citric acid cycle important for energy production. Through its activity in these pathways it interacts with proteins like aspartate aminotransferase (AST) affecting the balance of amino and keto acids in cells.\u003cbr\u003e\nAlanine transaminase levels indicate liver conditions such as hepatitis and liver cirrhosis. Elevated ALT activity suggests liver damage or inflammation. It also connects to conditions involving muscular injury. In these scenarios increased presence and activity of AST often accompany ALT providing a broader picture of the extent of tissue damage. Understanding the involvement of ALT in these pathways helps diagnose and monitor the progression of relevant health issues.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843649654953,"sku":"ab285264","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab285264","provider":"Iright","version":"1.0","type":"link"}