{"product_id":"abcam-ab286922","title":"Abcam, ab286922, Anti-SARS-CoV-2 nsp10 antibody - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal R1AB antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Recombinant fragment - SARS-CoV-2 samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR24836-8-8,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:SARS-CoV-2,\u003cbr\u003e\nApplications:I-ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSARS-CoV-2 nsp10 also known as non-structural protein 10 serves as a cofactor essential for the viral life cycle. It is about 15 kDa in mass and is expressed within infected host cells where the virus replicates. Mechanically nsp10 facilitates the formation of nsp10-nsp14 and nsp10-nsp16 complexes enhancing the exonuclease and methyltransferase activities of its partners. This function supports the viral RNA proofreading and mRNA cap methylation processes contributing to the clinical pathogenicity of the virus.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNsp10 plays a role in stabilizing nsp14 and nsp16 to help them perform their enzymatic functions effectively. These interactions involve complex formations required for maintaining the integrity of viral RNA synthesis. By coordinating with nsp14 nsp10 ensures the accurate proofreading of RNA to protect against mutations. In association with nsp16 it assists in mRNA cap methylation which is pivotal for evading host immune responses.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe interaction between nsp10 and its partner proteins integrate it into important viral replication and host immune evasion pathways. These pathways are critical for the SARS-CoV-2 lifecycle facilitating replication and transcription mechanisms. Through its role with nsp14 nsp10 contributes to the exonuclease pathway important for RNA error correction. Its association with nsp16 is significant in the methylation pathway preventing premature degradation of viral mRNA by the host cell’s immune system.\u003cbr\u003e\nNsp10 directly influences the progression of COVID-19 the disease caused by SARS-CoV-2. Its interactions with nsp14 and nsp16 affect the virus's ability to replicate efficiently and avoid detection by host defenses which can influence the severity of the disease. Understanding the dynamics of nsp10's interactions offers potential therapeutic targets for treating COVID-19 and managing the viral replication process to curb the disease's impact.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851278405801,"sku":"ab286922","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab286922","provider":"Iright","version":"1.0","type":"link"}