{"product_id":"abcam-ab287410","title":"Abcam, ab287410, Human FANCA knockout A549 cell line","description":"\u003cp\u003eSize: 1000000Cells \/ vial\u003cbr\u003e\nFANCA KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Next Generation Sequencing,Sanger Sequencing,Western blot,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-FANCA, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe FANCA protein also known as FAA protein is a critical component in the DNA repair mechanism. FANCA is approximately 162 kDa and shows widespread expression in the human body particularly in proliferating cells. This protein plays an essential role in maintaining genomic stability by participating in the repair of DNA interstrand crosslinks. FANCA's primary function involves its interaction with other proteins in the cell nucleus where DNA repair and replication processes occur.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nFANCA works as a part of the Fanconi anemia (FA) core complex involving around 13 proteins. This complex plays an important role in the FA DNA repair pathway facilitating the repair of interstrand DNA crosslinks. FANCA acts by orchestrating the recruitment and activation of downstream repair proteins which ensures the high-fidelity maintenance of the genetic material during cell division. Dysfunction in the FA complex where FANCA operates can lead to chromosomal instability and increased mutation rates.\u003cbr\u003e\nPathways\u003cbr\u003e\nFANCA functions prominently in the Fanconi anemia pathway and is also involved in the homologous recombination repair pathway. In the FA pathway FANCA works closely with related proteins like FANCB and FANCC to initiate the DNA repair process. FANCA's interaction with these proteins allows it to execute its repair role effectively ensuring cellular genomic integrity. These pathways are critical for protecting cells from DNA damage during replication and shielding them from cancer development.\u003cbr\u003e\nFANCA mutations are directly associated with Fanconi anemia a genetic disorder characterized by marrow failure cancer predisposition and various congenital abnormalities. Loss of FANCA function disrupts the repair pathway leading to cellular hypersensitivity to DNA-damaging agents. Additionally FANCA's impairment has been implicated in certain cancers like acute myeloid leukemia where its interaction with other FA proteins gets compromised leading to tumor progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845086531753,"sku":"ab287410","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab287410","provider":"Iright","version":"1.0","type":"link"}