{"product_id":"abcam-ab28760","title":"Abcam, ab28760, Anti-Triosephosphate isomerase antibody","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nGoat Polyclonal Triosephosphate isomerase antibody. Suitable for WB, IHC-P, Flow Cyt and reacts with Human, Mouse, Dog samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human TPI1 aa 200 to C-terminus.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Goat,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Dog, Human, Mouse,\u003cbr\u003e\nApplications:IHC-P, Flow Cyt, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Human TPI1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P60174,\u003cbr\u003e\nSpecificity:This antibody is expected to recognise both reported isoforms.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-This antibody was purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: 0.5% BSA, 0.5% Tris buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTriosephosphate isomerase also known as TPI is an enzyme that plays an important role in glycolysis and gluconeogenesis by catalyzing the reversible isomerization of dihydroxyacetone phosphate (DHAP) to glyceraldehyde 3-phosphate (G3P). It has a molecular mass of approximately 27 kDa. This enzyme is widely expressed in many tissues with high presence in red blood cells muscle and brain tissues due to their high energy demands. As a dimer enzyme it exhibits significant catalytic efficiency that supports cellular energy management.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe enzyme facilitates the efficient production of ATP by maintaining balance between DHAP and G3P vital for energy-metabolizing cells. Triosephosphate isomerase is not part of a larger complex itself but it significantly interacts with other enzymes in the glycolytic pathway. This interaction supports cellular respiration by ensuring a steady supply of intermediates needed for downstream processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nTriosephosphate isomerase is central to glycolysis and gluconeogenesis pathways. In glycolysis it works in tandem with other enzymes such as hexokinase and phosphofructokinase to breakdown glucose into pyruvate yielding ATP and NADH. It provides a critical link between glycolysis and energy production processes impacting the cellular metabolism and biochemical energy cycles. The enzyme also contributes to gluconeogenesis where it helps synthesize glucose from non-carbohydrate sources ensuring energy supply during fasting.\u003cbr\u003e\nMutations or deficiencies in triosephosphate isomerase can lead to conditions such as triosephosphate isomerase deficiency a rare autosomal recessive disorder characterized by progressive neurological dysfunction hemolytic anemia and muscle weakness. This deficiency arises from dysfunctional or unstable isomerase activity leading to an accumulation of DHAP. Additionally research has shown links between this enzyme and Alzheimer's disease where altered glucose metabolism is a common feature. Abnormal triosephosphate isomerase activity may interact with amyloid precursor protein processing in the disease pathology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848587366569,"sku":"ab28760","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab28760","provider":"Iright","version":"1.0","type":"link"}