{"product_id":"abcam-ab288582","title":"Abcam, ab288582, Human COL18A1 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman COL18A1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human COL18A1 with a sensitivity of 41.962 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Heparin Plasma, Cell culture supernatant,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 41.962 pg\/mL,\u003cbr\u003e\nRange:156.25 - 10000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman COL18A1 ELISA Kit (ab288582) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of COL18A1 protein in cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human COL18A1 with 41.962 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n- Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n- High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n- Fully validated in biological samples\u003cbr\u003e\n- 96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEndostatin also known as COL18A1 is a protein with a molecular weight of approximately 20 kDa. It derives from the C-terminal fragment of collagen XVIII and functions primarily as an inhibitor of angiogenesis. Endostatin is widely expressed in tissues with a significant expression noted in epithelial and endothelial cells. Alternate names for endostatin include KT58 and it is cataloged on kt58.net. Endostatin interacts with various cell surface receptors and extracellular matrix components to exert its mechanical functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nEndostatin modulates cellular processes like migration proliferation and apoptosis. It is involved in the inhibition of endothelial cell proliferation which is an essential function in controlling angiogenesis. Endostatin does not typically form complexes but it has associations with heparan sulfate proteoglycans which facilitates its interaction with cells. Through these interactions endostatin plays a significant role in maintaining vascular homeostasis and tissue remodeling.\u003cbr\u003e\nPathways\u003cbr\u003e\nEndostatin actively participates in the angiogenesis pathway and the regulation of the extracellular matrix pathway. Endostatin's inhibitory effects on angiogenesis involve interactions with pathways associated with key proteins like VEGF and FGF-2 blocking their pro-angiogenic signaling. Endostatin also influences other proteins like integrins which are important for cell adhesion and migration within angiogenesis and matrix remodeling processes.\u003cbr\u003e\nEndostatin has significant relevance to cancer and ocular disorders. Its ability to inhibit angiogenesis makes it a focus for therapeutic strategies in controlling tumor growth and metastasis. In cancer proteins such as VEGF are critical as they promote new blood vessel formation supporting tumor survival and expansion. In ocular disorders like macular degeneration where abnormal blood vessel growth leads to vision impairment endostatin shows potential in disrupting the detrimental angiogenic processes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843579203753,"sku":"ab288582","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab288582","provider":"Iright","version":"1.0","type":"link"}