{"product_id":"abcam-ab302760","title":"Abcam, ab302760, ARID1A ELISA Kit","description":"\u003cp\u003eSize: 96Tests\u003cbr\u003e\nARID1A ELISA Kit is a single-wash 90-min Simplestep used to quantify ARID1A with a sensitivity of 40.932 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture extracts,\u003cbr\u003e\nReacts with:Human, Mouse, Rat,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 40.932 pg\/mL,\u003cbr\u003e\nRange:1718.75 - 110000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nARID1A SimpleStep ELISA\u003cbr\u003e\nkit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ARID1A protein in cell extracts. Quantitate ARID1A with 273 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA\u003cbr\u003e\ntechnology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details. Our SimpleStep ELISA\u003cbr\u003e\ntechnology provides several benefits:\u003cbr\u003e\n-Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n-High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n-Fully validated in biological samples\u003cbr\u003e\n-96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA\u003cbr\u003e\nmicroplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nARID1A also known as BAF250A is a protein encoded by the ARID1A gene and forms part of the SWI\/SNF chromatin remodeling complex. The molecular weight of ARID1A is approximately 270 kDa. This protein is expressed in many tissues but higher expression is noted in the liver kidney and placenta. ARID1A contains an ARID (A-T Rich Interaction Domain) which allows it to bind DNA influencing transcription regulation significantly.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nARID1A impacts cellular functions through its role in the SWI\/SNF complex. This complex modulates chromatin structure and is integral to DNA accessibility which in turn controls gene expression. ARID1A's involvement helps in regulating transcription factors and affects processes such as cell growth and differentiation. Its function is necessary for maintaining proper cell proliferation and apoptosis balance.\u003cbr\u003e\nPathways\u003cbr\u003e\nARID1A plays a central role in transcriptional regulation and cell cycle control. It shows interaction with the tumor suppressor protein p53 and is implicated in the PI3K\/AKT signaling pathway. ARID1A's function in these pathways affects cellular responses to DNA damage and growth signals linking it closely to important cellular fate decisions.\u003cbr\u003e\nMutations or loss of function in ARID1A has been strongly associated with various cancers including ovarian and endometrial cancers. In these contexts ARID1A dysfunction often occurs alongside other proteins such as p53 contributing to tumorigenesis by deregulating tumor suppressive pathways. It also holds a potential connection to neurodevelopmental disorders due to its role in chromatin remodeling and gene expression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843568357545,"sku":"ab302760","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab302760","provider":"Iright","version":"1.0","type":"link"}