{"product_id":"abcam-ab303758","title":"Abcam, ab303758, Human Placental alkaline phosphatase (PLAP) ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Placental alkaline phosphatase (PLAP) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Placental alkaline phosphatase (PLAP) with a sensitivity of 20.466 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Cell culture media,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 20.466 pg\/mL,\u003cbr\u003e\nRange:31.25 - 2000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Placental alkaline phosphatase (PLAP) SimpleStep ELISA® kit (ab303758) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Placental alkaline phosphatase (PLAP) protein in serum, plasma (cit), plasma (hep), plasma (EDTA) and cell culture media. Quantitate Human Placental alkaline phosphatase (PLAP) with 20.466 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n-Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n-High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n-Fully validated in biological samples\u003cbr\u003e\n-96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPlacental alkaline phosphatase (PLAP) also known by names like alkaline phosphatase\/PLAP protein or ALPP functions as an enzyme that removes phosphate groups from molecules. It typically appears in tissues such as the placenta and is known to be active during pregnancy. PLAP has a molecular mass of approximately 66 kDa. Normally found expressed in the placenta this enzyme shares similarities with other alkaline phosphatases but is distinct due to its specific expression and function during gestation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPLAP shows importance in bone mineralization and fetal development. It participates in the hydrolysis of phosphate esters influencing nutrient availability and metabolism. PLAP is not a part of major protein complexes; however its enzymatic activity directly affects local phosphate concentrations which are critical for processes like bone formation and tissue regeneration during pregnancy.\u003cbr\u003e\nPathways\u003cbr\u003e\nPLAP expression and activity intersect significantly with phosphate metabolism and fetal development pathways. The alkaline phosphatase family plays central roles in these pathways and PLAP shares functions with other isoenzymes in the family such as the tissue nonspecific alkaline phosphatase. These enzymes regulate phosphate turnover and contribute to essential developmental processes.\u003cbr\u003e\nPLAP connects primarily to certain types of cancers and gestational disorders. Elevated levels of PLAP link to testicular germ cell tumors and ovarian cancer where it serves as a biomarker. In gestational trophoblastic disease PLAP expression provides valuable diagnostic information. Interactions with proteins like hCG (human chorionic gonadotropin) also offer insights into potential disease mechanisms especially in reproductive health challenges.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843578515625,"sku":"ab303758","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab303758","provider":"Iright","version":"1.0","type":"link"}