{"product_id":"abcam-ab305095","title":"Abcam, ab305095, TUBA1B Antibody Pair - BSA and Azide free","description":"\u003cp\u003eSize: 10 x 96Tests\u003cbr\u003e\nTUBA1B Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of TUBA1B.\u003cbr\u003e\nKey facts\u003cbr\u003e\nReacts with:Human, Mouse, Rat,\u003cbr\u003e\nAssay type:ELISA set,\u003cbr\u003e\nRange:0.08 - 20 ng\/mL,\u003cbr\u003e\nAssay Platform:Reagents\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman TUBA1B Antibody Pair - BSA and Azide free (ab305095) is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify human TUBA1B in sandwich ELISAs and many other pair-based applications.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nUse our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with \u0026lt;1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nThe pair can be used in variety of assays and platforms including but not limited to:\u003cbr\u003e\nSandwich ELISA\u003cbr\u003e\nFRET\/TR-FRET\/HTRF\u003cbr\u003e\nMeso Scale Discovery\u003cbr\u003e\nBead-based assays\u003cbr\u003e\nAlphaLISA\u003cbr\u003e\n\/AlphaScreen\u003cbr\u003e\nDELFIA\u003cbr\u003e\nimmunoassays\u003cbr\u003e\nand Single Molecule Counting (SMC™) immunoassays\u003cbr\u003e\nMultiplex\u003cbr\u003e\nOur antibody pairs are supplied in a carrier-free format that is conjugation-ready:\u003cbr\u003e\nBuffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.\u003cbr\u003e\nConcentration of ~1 mg\/ml as measured by the protein A280 method.\u003cbr\u003e\nWe can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.\u003cbr\u003e\nPairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.\u003cbr\u003e\nPlease note:\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.\u003cbr\u003e\nThe range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.\u003cbr\u003e\nWe guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.\u003cbr\u003e\nAntibody properties:\u003cbr\u003e\nCapture antibody:\u003cbr\u003e\nrecombinant rabbit monoclonal (unconjugated) – 100 µg\u003cbr\u003e\nDetector antibody:\u003cbr\u003e\nrecombinant rabbit monoclonal (unconjugated) - 100 µg\u003cbr\u003e\nConcentration\u003cbr\u003e\n: ~1 mg\/ml\u003cbr\u003e\nStorage buffer:\u003cbr\u003e\n100% PBS\u003cbr\u003e\nLiquid\u003cbr\u003e\nIsotype:\u003cbr\u003e\nRecombinant monoclonal antibodies offer several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free production\u003cbr\u003e\nMeso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.\u003cbr\u003e\nAlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.\u003cbr\u003e\nSimoa is a registered trademark of Quanterix, Inc.\u003cbr\u003e\nSMC is a registered trademark of Merck KGaA, Darmstadt, Germany.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTUBA1B also known as tubulin alpha-1B chain is a subunit of microtubules that plays a structural role in the cellular cytoskeleton. It has a molecular mass of about 50 kDa. TUBA1B distributes widely across various tissues with elevated expression in neuronal cells. It characterizes by its involvement in the assembly and stabilization of microtubules which are essential for maintaining cell shape promoting intracellular transport and facilitating chromosome segregation during cell division.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTUBA1B contributes significantly to the formation and function of the cytoskeletal microtubule network. It functions as part of the heterodimeric complex pairing with beta-tubulin to form the building blocks of microtubules. These structures are central to diverse cellular activities such as intracellular trafficking axonal transport in neurons and the migration and division of cells. In this implicit role TUBA1B influences the dynamics and stability of microtubule polymers impacting the cell’s ability to respond to mechanical and environmental stimuli.\u003cbr\u003e\nPathways\u003cbr\u003e\nTUBA1B is integral to the mitotic spindle assembly pathway and neuronal signaling. In the context of these pathways TUBA1B's interactions with proteins like dynein and kinesin facilitate its role in intracellular transport and spindle orientation during cell division. TUBA1B also participates in neuronal development pathways where its association with tau and other microtubule-associated proteins contributes to the formation of neuronal axons and dendrites.\u003cbr\u003e\nAlterations in TUBA1B have been linked to certain neurodevelopmental disorders and cancers such as lissencephaly and glioblastoma. In lissencephaly mutations in TUBA1B disrupt proper neuronal migration contributing to the abnormal brain development associated with the disorder. In glioblastoma TUBA1B's aberrant expression impacts microtubule dynamics aiding in cancer cell proliferation and resistance to therapies. These pathological conditions often involve interactions with other proteins including those found in the microtubule-associated protein family such as MAP2 and MAPT which further influence disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843598143657,"sku":"ab305095","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab305095","provider":"Iright","version":"1.0","type":"link"}