{"product_id":"abcam-ab305228","title":"Abcam, ab305228, Anti-HMGCS2 antibody [EPR26978-39] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal HMGCS2 antibody. Carrier free. Suitable for WB, Dot, IHC-P, ICC\/IF, Flow Cyt (Intra), IP and reacts with Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR26978-39,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:Dot, WB, Flow Cyt (Intra), ICC\/IF, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHMGCS2 also known as hydroxymethylglutaryl-CoA synthase 2 is an enzyme involved in ketogenesis. It catalyzes the conversion of acetyl-CoA to HMG-CoA an essential step in the production of ketone bodies. This enzyme has a molecular mass of approximately 57 kDa. HMGCS2 is mainly expressed in the liver and is located within the mitochondria where it works under controlled metabolic conditions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nHMGCS2 activates the ketogenesis process which generates ketone bodies such as acetoacetate and β-hydroxybutyrate that serve as alternative energy sources when glucose is scarce. This enzyme is not known to be part of any larger protein complex. During fasting states or prolonged exercise the liver uptakes fatty acids and converts them into ketone bodies through HMGCS2 activity providing energy especially for organs like the brain and muscles.\u003cbr\u003e\nPathways\u003cbr\u003e\nHMGCS2 functions in the ketogenesis and lipid metabolism pathways. It works alongside enzymes like HMG-CoA lyase which further processes HMG-CoA into acetoacetate a precursor for other ketone bodies. The interplay of HMGCS2 with fatty acid oxidation allows it to regulate energy homeostasis coordinating with enzymes such as carnitine palmitoyltransferase I which aids in transporting fatty acids into mitochondria for oxidation.\u003cbr\u003e\nHMGCS2 malfunctions or deficiencies relate to disorders such as hypoketotic hypoglycemia and hepatic encephalopathy. Disrupted activity can affect energy balance particularly during fasting impacting glucose homeostasis. Alterations in HMGCS2 expression or function can connect with proteins like medium-chain acyl-CoA dehydrogenase which plays roles in fatty acid metabolism disorders further complicating the metabolic landscape when HMGCS2 is impaired.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848562856105,"sku":"ab305228","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab305228","provider":"Iright","version":"1.0","type":"link"}