{"product_id":"abcam-ab307477","title":"Abcam, ab307477, Recombinant Human Liver Arginase Protein (His tag)","description":"\u003cp\u003eSize: 10µg \/ 50µg \/ 100µg\u003cbr\u003e\nRecombinant Human Liver Arginase Protein (His tag) is a Human Full Length protein, in the 1 to 322 aa range, expressed in HEK 293 cells, with \u0026gt;95%, \u0026lt;0.005 EU\/µg endotoxin level, suitable for HPLC, Mass Spec, SDS-PAGE.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% HPLC,\u003cbr\u003e\nEndotoxin level:\u0026lt;0.005 EU\/µg,\u003cbr\u003e\nExpression system:HEK 293 cells,\u003cbr\u003e\nTags:His tag C-Terminus,\u003cbr\u003e\nApplications:HPLC, SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:No,\u003cbr\u003e\nAccession:P05089,\u003cbr\u003e\nAnimal free:Yes,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nReconstitution:Reconstitute in PBS,\u003cbr\u003e\nStorage buffer:pH: 7.4Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nArginase also known as arginase-1 (ARG1) and arginase-2 (ARG2) depending on the isoform is an important enzyme in the urea cycle. Its main function is to catalyze the hydrolysis of arginine to ornithine and urea. The molecular mass of arginase varies between the isoforms with ARG1 typically around 35 kDa. Arginase is expressed widely including in the liver kidneys and red blood cells for ARG1 whereas ARG2 is more tissue-specific found particularly in the kidneys brain and small intestine.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nArginase plays a significant role in nitrogen metabolism and has a direct impact on ammonia detoxification. Arginase functions as a homotrimer facilitating the conversion of toxic ammonia into urea which the body excretes safely. The liver is the main site of the urea cycle and where ARG1 predominantly operates while ARG2 functions in mitochondrial roles associated with regulating arginine levels in other tissues. Additionally arginase impacts nitric oxide (NO) production as it competes with nitric oxide synthase (NOS) for their common substrate L-arginine.\u003cbr\u003e\nPathways\u003cbr\u003e\nArginase is an important component of the urea cycle and plays a part in arginine and proline metabolism. Through its function in these pathways arginase affects several physiological processes. It competes with nitric oxide synthase for L-arginine influencing NO production which is involved in vasodilation and immune response regulation. Arginase's function in the urea cycle connects it to other urea cycle enzymes like ornithine transcarbamylase (OTC) and carbamoyl phosphate synthetase I (CPS1).\u003cbr\u003e\nElevated arginase activity has links to certain vascular disorders such as hypertension due to reduced nitric oxide production. Arginase also plays a role in cancer as tumor cells often display increased arginase activity which depletes local arginine affecting T-cell function and aiding tumor immune evasion. These conditions often involve interactions with proteins like nitric oxide synthase which shares L-arginine as a substrate with arginase leading to potential therapeutic targets in managing these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843831386281,"sku":"ab307477","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab307477","provider":"Iright","version":"1.0","type":"link"}