{"product_id":"abcam-ab307632","title":"Abcam, ab307632, Recombinant Human ICOS Ligand\/ICOSL Protein (Fc Chimera) (Active)","description":"\u003cp\u003eSize: 10µg \/ 50µg \/ 100µg\u003cbr\u003e\nRecombinant Human ICOS Ligand\/ICOSL Protein (Fc Chimera) (Active) is a Human Fragment protein, in the 19 to 256 aa range, expressed in HEK 293 cells, with \u0026gt;95%, \u0026lt;0.005 EU\/µg endotoxin level, suitable for SDS-PAGE, HPLC, Mass Spec.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% HPLC,\u003cbr\u003e\nEndotoxin level:\u0026lt;0.005 EU\/µg,\u003cbr\u003e\nExpression system:HEK 293 cells,\u003cbr\u003e\nTags:Fc tag C-Terminus,\u003cbr\u003e\nApplications:HPLC, SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Loaded Human ICOS Ligand, Human Fc Tag on Protein A Biosensor can bind Human ICOS, His Tag with an affinity constant of 117 nM as determined in BLI assay (GatorBio Prime).,\u003cbr\u003e\nAccession:O75144,\u003cbr\u003e\nAnimal free:Yes,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nReconstitution:Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.,\u003cbr\u003e\nStorage buffer:pH: 7.4Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe ICOS Ligand also known as ICOSL B7-H2 and HK53 is a critical component in immune system function. This protein with a molecular mass of approximately 65 kDa is expressed on professional antigen-presenting cells such as dendritic cells macrophages and B cells. It plays a significant mechanical role in the immune response by engaging with its counter receptor the ICOS protein present on the surface of activated T cells. This interaction supports the regulation of T cell activity influencing proliferation and cytokine production.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nICOSL contributes to the modulation of immune responses. By forming a complex with the active ICOS protein ICOSL is involved in the co-stimulation of T cells. This process is important for the development of T-cell-dependent immune responses and helps in promoting the survival and differentiation of T cells. Together the ICOS ligand and ICOS protein create a dynamic interaction that balances immune activation and tolerance which is essential for maintaining immune homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe ICOS-ICOSL interaction plays an important role in the T-cell receptor (TCR) signaling pathway. This pathway is fundamental for the activation of T cells and subsequent immune response. ICOSL also connects to the PI3K\/Akt pathway where it impacts cell survival and proliferation. ICOS and the PI3K\/Akt pathway work together to influence various aspects of immune regulation and adaptive immunity.\u003cbr\u003e\nICOSL has a notable impact on autoimmune diseases and cancer. Its function in immune modulation when dysregulated can contribute to autoimmune conditions such as systemic lupus erythematosus. In this context the abnormal activation of the ICOS protein may exaggerate immune responses leading to pathology. In cancer high expression of ICOSL can support tumor progression by affecting the immune surveillance capability. These associations reveal ICOSL's role in balancing immune activation and inhibition which is important in the context of disease.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843842691241,"sku":"ab307632","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab307632","provider":"Iright","version":"1.0","type":"link"}