{"product_id":"abcam-ab307714","title":"Abcam, ab307714, Anti-Gli3 antibody [EPR26837-58]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nRabbit Recombinant Monoclonal GLI3 antibody. Suitable for WB, IP and reacts with Human, Mouse samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR26837-58,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human, Mouse,\u003cbr\u003e\nApplications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGli3 also known as GLI Family Zinc Finger 3 is a transcription factor that plays a significant role in the Hedgehog signaling pathway. Gli3 functions as both an activator and a repressor depending on its post-translational modification state. It has a molecular mass of approximately 172 kDa. Mainly expressed in the limb buds central nervous system and other embryonic tissues Gli3 influences the development of various structures during embryogenesis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGli3 is essential for proper organ and tissue development. It associates with other proteins including the Suppressor of Fused (SUFU) to form complexes that regulate target gene expression. Gli3 interacts with GLI1 and GLI2 all of which are part of the Gli family of transcription factors. These interactions modulate various developmental processes by controlling target gene transcription ensuring tissues develop correctly.\u003cbr\u003e\nPathways\u003cbr\u003e\nGli3 is an important player in the Hedgehog signaling pathway and also impacts a secondary pathway the Wnt signaling pathway. Gli3 acts in coordination with the Sonic Hedgehog (SHH) protein regulating developmental gene expression. SHH binds to the Patched receptor affecting Gli3's function as a transcriptional repressor or activator. By these pathways Gli3 ensures proper cellular communication and structural formation.\u003cbr\u003e\nMutations or dysregulation of Gli3 are connected to two major conditions: Pallister-Hall Syndrome and Greig Cephalopolysyndactyly Syndrome. Both conditions involve limb malformations and other developmental defects due to abnormal GLI3 function. Furthermore Gli3 dysregulation links to abnormal activity of SUFU and GLI2 contributing to the pathogenesis of these syndromes. Understanding these interactions can provide critical insights into therapeutic strategies for related disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46850335899817,"sku":"ab307714","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab307714","provider":"Iright","version":"1.0","type":"link"}