{"product_id":"abcam-ab308342","title":"Abcam, ab308342, Anti-p53 R175H Mutant antibody [HL1129]","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nRabbit Recombinant Monoclonal P53 mutated R175H antibody. Carrier free. Suitable for IHC-P, ICC\/IF, WB and reacts with Human, Transfected cell line samples. Immunogen corresponding to Synthetic Peptide within Human Cellular tumor antigen p53 mutated R175H.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:HL1129,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, IHC-P, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Human Cellular tumor antigen p53 mutated R175H. The exact immunogen used to generate this antibody is proprietary information.P04637\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMutant p53 also known as tumor protein p53 or TP53 is a variant of the p53 protein which usually acts as a guardian of the genome. The regular p53 functions as a transcription factor and has a molecular mass of approximately 53 kDa. It is expressed in various tissues throughout the body. In its mutant form p53 loses its ability to ensure genomic stability and becomes dysfunctional leading to abnormal cellular processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMutant p53 impacts several cellular mechanisms beyond its usual role in DNA repair and cell cycle regulation. It can form part of various protein complexes that disrupt normal cellular functions promoting oncogenic transformation. Mutant p53 often accumulates to higher levels within the cell compared to its wild-type counterpart leading to gain-of-function properties which contribute to cancer development.\u003cbr\u003e\nPathways\u003cbr\u003e\nMutant p53 interacts with many cellular pathways such as the p53 signaling pathway and apoptosis regulation. It influences the pathways by altering interactions with other proteins like MDM2 and RB1 modifying normal cellular responses to stress and damage. These modifications can disrupt regular apoptotic processes and cell cycle checkpoints further assisting in tumorigenesis.\u003cbr\u003e\nMutant p53 is strongly linked with various cancers including breast and colorectal cancers. Mutations in p53 are among the most common genetic alterations observed in cancerous cells and they enable tumors to bypass growth suppression and evade apoptosis. Through cancerous pathways mutant p53 becomes interconnected with proteins like BRCA1 in breast cancer and APC in colorectal cancer promoting disease progression and resistance to standard therapies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46856258879657,"sku":"ab308342","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab308342","provider":"Iright","version":"1.0","type":"link"}