{"product_id":"abcam-ab308529","title":"Abcam, ab308529, Anti-SLC16A3\/MCT 4 antibody [EPR28177-30] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal SLC16A3\/MCT 4 antibody. Carrier free. Suitable for WB, IHC-P, ICC\/IF, Flow Cyt (Intra), IHC-Fr and reacts with Human, Mouse, Rat samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR28177-30,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human, Mouse, Rat,\u003cbr\u003e\nApplications:Flow Cyt (Intra), IHC-Fr, ICC\/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSLC16A3 also known as MCT4 or MCT-4 is a member of the monocarboxylate transporter family. It is responsible for the transport of lactate and other monocarboxylates across cell membranes. Typically the molecular weight of MCT4 is approximately 45-50 kDa. This transporter is most abundant in tissues with high glycolytic activity including muscle and certain types of tumors where it facilitates the export of lactate produced during anaerobic metabolism to maintain intracellular pH balance and energy production efficiency.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe transport function of SLC16A3\/MCT4 is important for maintaining the energy equilibrium in cells that rely heavily on glycolysis. MCT4 does not function independently; it associates with ancillary proteins like CD147\/Basigin which is essential for its correct localization to the plasma membrane. This association enhances the transport efficiency of lactate making MCT4 an important player in the lactate shuttle system a mechanism that allows for effective energy utilization in muscle fibers and tumor cells and significantly contributes to the regulation of the tissue microenvironment.\u003cbr\u003e\nPathways\u003cbr\u003e\nSLC16A3\/MCT4 plays a significant role in metabolic pathways particularly the glycolytic pathway by facilitating lactate efflux. The transporter is also implicated in the Cori cycle where lactate is shuttled from muscles to the liver for gluconeogenesis. Its functions overlap with those of other monocarboxylate transporters such as MCT1 which generally handles the import of lactate in oxidative tissues. Together these transporters maintain homeostasis of lactate levels in various tissues preventing lactate accumulation.\u003cbr\u003e\nAlterations in SLC16A3\/MCT4 expression or function are associated with cancer and anaerobic exercise intolerance. In cancer overexpression of MCT4 correlates with aggressive tumor behavior due to its role in lactate export aiding in the acidic tumor microenvironment development which favors cancer cell survival and invasion. Additionally interactions with proteins like CD147 are thought to modulate tumor progression. In exercise intolerance impaired lactate transport leads to muscle fatigue and reduced exercise capacity highlighting the critical role MCT4 plays in normal metabolic responses during intense physical activity.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851619127465,"sku":"ab308529","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab308529","provider":"Iright","version":"1.0","type":"link"}