{"product_id":"abcam-ab310799","title":"Abcam, ab310799, Recombinant Human IL-18 Protein (His tag) (Active)","description":"\u003cp\u003eSize: 10µg \/ 50µg \/ 100µg\u003cbr\u003e\nRecombinant Human IL-18 Protein (His tag) (Active) is a Human Full Length protein, in the 37 to 193 aa range, expressed in Escherichia coli, with \u0026gt;95%, \u0026lt;0.1 EU\/mg endotoxin level, suitable for Mass Spec, SDS-PAGE, HPLC, Cell Culture.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% HPLC,\u003cbr\u003e\nEndotoxin level:\u0026lt;0.1 EU\/mg,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:His tag C-Terminus,\u003cbr\u003e\nApplications:HPLC, Cell Culture, SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Fully biologically active determined by the dose dependent NF-κB and AP-1 pathway activation of human embryonic kidney (HEK)- 293 cells overexpressing IL-18R and IL-18RAP inducible SEAP reporter genes. Results obtained with Invivogen HEK BlueTM IL-18 cells (Cat code., hkb-hmil18). ED50 ≤ 0.03 ng\/ml, corresponding to a specific activity of 3.3 x 107 units\/mg.,\u003cbr\u003e\nAccession:Q14116,\u003cbr\u003e\nAnimal free:Yes,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nReconstitution:Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.,\u003cbr\u003e\nStorage buffer:pH: 7.4Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843825094825,"sku":"ab310799","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab310799","provider":"Iright","version":"1.0","type":"link"}