{"product_id":"abcam-ab312861","title":"Abcam, ab312861, Anti-VEGFA antibody [EPR24630-77] - BSA and Azide free (Capture)","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal VEGFA antibody. Carrier free. Suitable for sELISA and reacts with Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR24630-77,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nSandwich ELISA\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nVEGF or Vascular Endothelial Growth Factor is a signaling protein that plays an essential role in blood vessel formation. Known also as VEGFA its molecular mass is approximately 45 kDa. VEGF is expressed in a variety of tissues especially those with high angiogenic activity such as endothelial cells macrophages and platelets. This protein activates receptors on the surface of endothelial cells triggering a cascade of intracellular events that lead to cellular proliferation migration and increased vascular permeability.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein regulates angiogenesis the process through which new blood vessels form from pre-existing vasculature. It interacts with specific tyrosine kinase receptors such as VEGFR-1 and VEGFR-2. VEGF is not part of a multi-protein complex but it can form homodimers which significantly enhances its activity. It ensures proper vascularization during development and wound healing by directing the growth and branching of vessels. This makes it an important factor in supplying oxygen and nutrients to tissues.\u003cbr\u003e\nPathways\u003cbr\u003e\nVEGF is primarily involved in the VEGF signaling and PI3K\/AKT pathways. These pathways allow it to mediate endothelial cell function and survival. In the VEGF signaling pathway it associates closely with members like VEGFR-2 and PLCγ1 which activate further signal transduction needed for angiogenesis. The PI3K\/AKT pathway linked with cell proliferation and survival also interacts with VEGF connecting it to other important proteins like AKT and mTOR highlighting its significant role in cellular responses to environmental stimuli.\u003cbr\u003e\nVEGF has a strong association with cancer and age-related macular degeneration. In cancer overexpression of VEGF leads to pathological angiogenesis providing tumors with the blood supply needed for growth and metastasis. Tumor cells through hypoxia-inducible factors (HIF) upregulate VEGF expression correlating with cancer progression. In age-related macular degeneration abnormal blood vessel growth influenced by VEGF contributes to visual impairment and blindness further implicating the protein in ocular diseases. Targeted therapies often aim at inhibiting VEGF activity to control the disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851281158313,"sku":"ab312861","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab312861","provider":"Iright","version":"1.0","type":"link"}