{"product_id":"abcam-ab314530","title":"Abcam, ab314530, Recombinant mouse Claudin18 protein - Active (His tag)","description":"\u003cp\u003eSize: 20µg \/ 100µg\u003cbr\u003e\nRecombinant mouse Claudin18 protein - Active (His tag) is a Mouse Full Length protein, in the 1 to 264 aa range, expressed in Mammalian, \u0026lt; 1 EU\/µg endotoxin level, suitable for WB, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nEndotoxin level:\u0026lt; 1 EU\/µg,\u003cbr\u003e\nExpression system:Mammalian,\u003cbr\u003e\nTags:His tag N-Terminus,\u003cbr\u003e\nApplications:WB, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Measured by its binding ability in a functional ELISA. AB314530 at 5 μg\/mL can bind an Anti-CLDN18.2 recombinant antibody, the EC50 is 6.115-11.01 ng\/mL.,\u003cbr\u003e\nAccession:P56857,\u003cbr\u003e\nAnimal free:Yes,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Mouse,\u003cbr\u003e\nReconstitution:Reconstitute in water,\u003cbr\u003e\nStorage buffer:pH: 7.4 - 8Constituents: 6% Trehalose, 0.14% PBS\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg\/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC\/-80degC. Our default final concentration of glycerol is 50%.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, -80°C, Appropriate long-term storage conditions--20°C, -80°C, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nClaudin-18 often noted as Claudin18 or CLDN18 is a tight junction protein belonging to the Claudin family. The protein comes in different isoforms including Claudin 18.2 each having a distinct function and expression pattern. With an estimated molecular mass of approximately 24 kDa it is mainly expressed in epithelial cells of the stomach and specific lung epithelia. Researchers utilize it frequently in immunohistochemistry (IHC) studies often referred to as CLDN18 IHC for its role in maintaining cell-to-cell adhesion in these tissues.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nClaudin-18.2 integrates itself into a tight junction complex contributing to the selective permeability barrier in epithelial tissues. This barrier is essential for maintaining tissue homeostasis and protecting underlying tissue from various external factors. It plays a significant role in ion selectivity and barrier assurance of the epithelial cells-gastrointestinal site especially. The regulated expression and function of Claudin 18 are critical for effective epithelial barrier function.\u003cbr\u003e\nPathways\u003cbr\u003e\nClaudin-18 is involved in pathways concerning epithelial morphogenesis and integrity. It aligns with occludins and other claudins to control paracellular transport and cellular integrity within these pathways. Through its association with proteins such as ZO-1 Claudin-18 ensures proper cell signaling and tight junction formation. Its regulation in epithelial cells contributes significantly to pathways that sustain cell structure and prevent uncontrolled leakage of solutes.\u003cbr\u003e\nThe altered expression of Claudin-18.2 is closely linked to gastric and lung cancers. Abnormal levels can disrupt the epithelial barrier contributing to tumor progression and metastasis. In gastric cancer altered Claudin-18 couples with overexpression of other proteins such as HER2 playing a role in the disease's aggressiveness. Researchers consider it a potential target for therapeutic interventions making Claudin-18.2 purchase or acquisition relevant for studies oriented toward cancer diagnostics and treatment.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843905179817,"sku":"ab314530","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab314530","provider":"Iright","version":"1.0","type":"link"}