{"product_id":"abcam-ab315391","title":"Abcam, ab315391, Anti-TCF7 antibody [EPR28579-32] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal TCF7 antibody. Carrier free. Suitable for WB, IP, IHC-P, ICC\/IF, mIHC, Flow Cyt (Intra) and reacts with Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR28579-32,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IP, IHC-P, ICC\/IF, Flow Cyt (Intra), mIHC, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:This antibody does not recognize the dominant negative isoforms of TCF1\/TCF7 lacking the amino-terminal β-catenin binding domain.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab315391 is the carrier-free version of\u003cbr\u003e\nab315390\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTCF7 also known as TCF1 is a transcription factor belonging to the TCF\/LEF family with a molecular mass of approximately 54 kDa. TCF7 plays a significant mechanical role in binding to DNA and regulating gene expression in cell nuclei. It interacts with beta-catenin to control the transcription of target genes. This protein is expressed in various tissues including the thymus and other parts of the immune system as well as the intestines.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTCF7 regulates cell fate decisions and differentiation specifically in the T-cell lineage. It is part of a protein complex with beta-catenin and acts as a transcriptional activator. TCF7 influences the development and function of T-cells playing a critical role in adaptive immune response. It contributes to the maintenance of stem cell properties in various tissues impacting processes like cell proliferation and survival.\u003cbr\u003e\nPathways\u003cbr\u003e\nTCF7 is a vital component of the Wnt\/beta-catenin signaling pathway which is essential for regulating gene expression in various cellular processes. This pathway maintains homeostasis in cell proliferation differentiation and stem cell renewal. Within this signaling network beta-catenin an important mediator partners with TCF7 to modulate target gene transcription. TCF7 also interacts with other proteins such as LEF1 which similarly participates in the transcription regulation vital for cellular development.\u003cbr\u003e\nTCF7 associates with several pathological conditions including cancers and immunodeficiencies. Abnormal activation of the Wnt\/beta-catenin pathway involving TCF7 contributes to the development of colon cancer by promoting unregulated cell division. TCF7 mutations have been linked to immunodeficiency disorders given its critical role in T-cell development. These mutations may disrupt normal T-cell formation leading to impaired immune responses.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851609165993,"sku":"ab315391","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab315391","provider":"Iright","version":"1.0","type":"link"}