{"product_id":"abcam-ab315801","title":"Abcam, ab315801, Anti-CYP51A1\/CYP51 antibody [EPR27379-150] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal CYP51A1\/CYP51 antibody. Carrier free. Suitable for mIHC, IHC-P, WB, Flow Cyt (Intra), IP, ICC\/IF and reacts with Human, Mouse, Rat samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR27379-150,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human, Mouse, Rat,\u003cbr\u003e\nApplications:mIHC, ICC\/IF, Flow Cyt (Intra), IHC-P, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab315801 is the carrier-free version of\u003cbr\u003e\nab315800\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCYP51A1 also known as CYP51 is a cytochrome P450 enzyme. It plays an important mechanical role in cholesterol biosynthesis specifically catalyzing the demethylation of lanosterol. This enzyme has a molecular mass of approximately 55 kDa. CYP51A1 is expressed in multiple tissues including the liver testes and adrenal glands reflecting its widespread functional importance within the body.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCYP51A1 is essential in the production of sterols such as cholesterol which are fundamental components of cell membranes. It does not operate alone; rather it forms part of an enzymatic complex involved in lipid homeostasis. By participating in this complex CYP51A1 influences cell membrane integrity fluidity and signaling.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe activity of CYP51A1 integrates into the cholesterol biosynthesis pathway where it serves a critical role in the downstream conversion of lanosterol to cholesterol. Its action influences the pathway alongside other key enzymes like HMG-CoA reductase. Additionally the enzyme participates in broader lipid metabolic pathways linking with proteins such as squalene synthase that also contribute to sterol production.\u003cbr\u003e\nCYP51A1's activity associates with certain lipid metabolism disorders including congenital adrenal hyperplasia and Smith-Lemli-Opitz syndrome. These conditions often involve disrupted cholesterol synthesis where CYP51A1’s malfunction can play a part. Moreover CYP51A1 connects to liver function through its interaction with proteins such as sterol regulatory element binding proteins (SREBPs) which regulate cholesterol homeostasis and can be associated with liver diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851616112809,"sku":"ab315801","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab315801","provider":"Iright","version":"1.0","type":"link"}