{"product_id":"abcam-ab315887","title":"Abcam, ab315887, Anti-Puromycin antibody [EPR27218-173]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-Puromycin antibody [EPR27218-173] (ab315887) is a rabbit monoclonal antibody detecting Puromycin in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC\/IF . - Biophysical QC for unrivalled batch-batch consistency\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR27218-173,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nApplications:IP, Flow Cyt (Intra), WB, ICC\/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-Puromycin antibody [EPR27218-173] (ab315887) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF).\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR27218-173] also available for your convenience: ab315887, Carrier free -\u003cbr\u003e\nab315888\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab322729\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab323262\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPuromycin is a well-known antibiotic that acts by disrupting protein synthesis. It mimics the structure of an aminoacyl-tRNA leading to premature termination of the growing polypeptide chain during translation. Puromycin is small with a molecular mass of approximately 471 daltons. It interacts mainly within the cytoplasm where it can incorporate into the peptide chain due to its similarity to the 3' end of the aminoacyl-tRNA. Puromycin does not have a specific expression site like endogenous proteins because it is not naturally occurring in animals; rather it is used experimentally.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe function of puromycin is to interfere with protein synthesis by targeting ribosomes. It binds to the ribosomal A site leading to the release of incomplete polypeptides. Puromycin is not part of a complex but establishes a strong interaction with ribosomes found in prokaryotic and eukaryotic cells. This interaction gives it unique utility in experiments that require inhibition of translation such as studies involving protein turnover and synthesis rates.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe action of puromycin strongly impacts protein synthesis pathways. It inhibits the elongation stage of the translation pathway by mimicking an aminoacyl-tRNA. Puromycin does not involve complex pathways itself but its effects can influence other proteins involved in translation such as elongation factors and the ribosomal subunits. This makes puromycin useful in basic scientific research to study how cells control protein synthesis.\u003cbr\u003e\nPuromycin's ability to inhibit translation links it to studies on cancer and neurodegenerative diseases. Researchers use puromycin to explore how disrupting protein synthesis can affect cancer cell growth. It serves as a model for understanding toxins that affect similar pathways in neurodegenerative disorders. Through these studies connections have been made between puromycin's action and proteins involved in these diseases such as oncogenes and neuromodulatory proteins which are key to understanding disease progression and developing potential treatments.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844155330729,"sku":"ab315887","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab315887","provider":"Iright","version":"1.0","type":"link"}