{"product_id":"abcam-ab316901","title":"Abcam, ab316901, Human Syntaxin 1B ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Syntaxin 1B ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Syntaxin 1B with a sensitivity of 16.949 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate, Tissue Extracts,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 16.949 pg\/mL,\u003cbr\u003e\nRange:62.5 - 4000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Syntaxin 1B SimpleStep ELISA\u003cbr\u003e\nkit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Syntaxin 1B protein in Cell Lysate, Tissue extracts. Quantitate Human Syntaxin 1B with 16.949 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA\u003cbr\u003e\ntechnology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details. Our SimpleStep ELISA\u003cbr\u003e\ntechnology provides several benefits:\u003cbr\u003e\n-Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n-High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n-Fully validated in biological samples\u003cbr\u003e\n-96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA\u003cbr\u003e\nmicroplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSyntaxin 1B often abbreviated as STX1B is an essential SNARE protein with a mass of approximately 33 kDa. This protein is mainly expressed in neurons and tissues with neural characteristics indicating its prominent role in the nervous system. Mechanically Syntaxin 1B facilitates the docking and fusion of synaptic vesicles with the presynaptic membrane. Through this mechanism it aids in the release of neurotransmitters which is fundamental for synaptic transmission and communication between neurons.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein functions as a core component of the SNARE complex comprising Syntaxin 1B SNAP-25 and VAMP2. The formation of this complex brings the synaptic vesicle and presynaptic membrane into close proximity creating the condition for vesicle fusion. Syntaxin 1B plays a significant role in synaptic efficiency and plasticity impacting processes such as learning and memory.\u003cbr\u003e\nPathways\u003cbr\u003e\nSyntaxin 1B is integral to the neurotransmitter release pathway and exocytosis pathways. It closely interacts with proteins such as SNAP-25 and VAMP2 both of which are critical for the proper execution of synaptic vesicle release. Within these pathways Syntaxin 1B assists in the regulated release of chemical messengers ultimately influencing neuronal communication and overall neural network activity.\u003cbr\u003e\nMutations or dysfunctions in Syntaxin 1B have links to neurological conditions such as epilepsy and intellectual disability. Research indicates that abnormalities in Syntaxin 1B might disrupt synaptic transmission potentially through altered interactions with proteins like SNAP-25. These disruptions can lead to erratic neuronal firing patterns or inadequate synaptic connectivity contributing to the pathophysiology observed in these disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843636449449,"sku":"ab316901","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab316901","provider":"Iright","version":"1.0","type":"link"}