{"product_id":"abcam-ab316909","title":"Abcam, ab316909, Human CD16 ELISA Kit- Extracellular","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman CD16 ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human CD16 with a sensitivity of 8.667 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Serum, EDTA Plasma, Citrate plasma, Heparin Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 8.667 pg\/mL,\u003cbr\u003e\nRange:19.531 - 1250 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman CD16 SimpleStep ELISA\u003cbr\u003e\nkit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD16 protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma. Quantitate Human CD16 with 8.667 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA\u003cbr\u003e\ntechnology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details. Our SimpleStep ELISA\u003cbr\u003e\ntechnology provides several benefits:\u003cbr\u003e\n-Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n-High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n-Fully validated in biological samples\u003cbr\u003e\n-96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA\u003cbr\u003e\nmicroplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCD16 often known as Fc gamma receptor III (FcγRIII) comes in two isoforms: CD16a and CD16b. CD16a is a transmembrane protein with a mass of about 50-65 kDa while CD16b a glycosylphosphatidylinositol (GPI)-anchored receptor holds a mass near 48-61 kDa. CD16 is expressed on natural killer (NK) cells monocytes macrophages and neutrophils depending on the variant. It serves as a low-affinity receptor for the Fc portion of IgG antibodies mediating essential immune functions like antibody-dependent cellular cytotoxicity (ADCC).\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCD16 plays an important role in immune response regulation and is part of the larger IgG receptor family. As a member of the receptor complex CD16 enables effector cells to identify and eliminate IgG-bound pathogens or diseased cells. The differential expression of CD16a and CD16b provides distinct roles in immune signaling. CD16a expressed on NK cells is involved in ADCC by enabling these cells to recognize and destroy antibody-coated target cells. CD16b predominantly found on neutrophils influences the phagocytosis process and aids in inflammatory response modulation.\u003cbr\u003e\nPathways\u003cbr\u003e\nCD16 integrates into significant immune response pathways. In the ADCC pathway CD16a interacts with other proteins like FcεRI gamma-chain to trigger cytotoxic activities in NK cells. The CD16b isoform participates in neutrophil-mediated immune responses connecting to downstream signaling molecules that modulate inflammation and pathogen clearance. These interactions ensure an effective immune response and maintain homeostasis by removing pathogenic threats.\u003cbr\u003e\nCD16 plays vital roles in immune-related conditions like autoimmune diseases and chronic inflammation. Aberrant CD16 expression or function is associated with autoimmune conditions such as rheumatoid arthritis where altered CD16a and CD16b expressions contribute to the pathology. Furthermore CD16 links to systemic lupus erythematosus (SLE) interacting with proteins like complement factors C3 and C4 influencing disease progression and severity. Understanding CD16's role offers insights into these disorders and informs potential therapeutic strategies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843642151081,"sku":"ab316909","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab316909","provider":"Iright","version":"1.0","type":"link"}