{"product_id":"abcam-ab317574","title":"Abcam, ab317574, Anti-ABCB4 antibody [EPR28774-92] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal ABCB4 antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Mouse, Rat, Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR28774-92,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab317574 is the carrier-free version of\u003cbr\u003e\nab317573\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nABCB4 also known as MDR3 is a member of the ATP-binding cassette (ABC) transporter family with a molecular weight of approximately 140 kDa. This protein is mainly expressed in the canalicular membrane of hepatocytes in the liver. Its primary mechanical function involves the translocation of phosphatidylcholine from the inner to the outer leaflet of the hepatocyte membrane into bile.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis phospholipid translocation is essential for the formation of bile that is non-toxic and aids in digestion. ABCB4 does not function alone; it operates as a part of a heterocomplex with other proteins that contribute to bile formation and secretion. The absence or dysfunction of this target results in imbalanced bile composition which can negatively impact liver function.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe ABCB4 protein plays a significant role in the enterohepatic circulation of bile acids. It interacts closely with the bile salt export pump (BSEP) to facilitate the secretion of bile acids into bile. This protein is also linked to the phospholipid transport exact in the hepatic pathways collaborating with other ABC transporters such as ABCB11 underpinning the finely tuned processes that maintain hepatic homeostasis.\u003cbr\u003e\nMutations or deficiencies in ABCB4 are commonly associated with progressive familial intrahepatic cholestasis type 3 (PFIC3) and intrahepatic cholestasis of pregnancy (ICP). Defects in the function of this protein lead to toxic bile accumulation causing cholestasis. Other related proteins such as ABCB11 and ATP8B1 may also influence the occurrence of these disorders due to their overlapping roles in hepatobiliary transport processes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46852547674281,"sku":"ab317574","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab317574","provider":"Iright","version":"1.0","type":"link"}