{"product_id":"abcam-ab317859","title":"Abcam, ab317859, Anti-Collybistin antibody [EPR28024-40] - BSA and Azide free (Detector)","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal ARHGEF9 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR28024-40,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human, Mouse, Rat,\u003cbr\u003e\nApplications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab317859 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Collybistin. The recommended pair for sandwich ELISA is:\u003cbr\u003e\nCapture:\u003cbr\u003e\nab317858\u003cbr\u003e\n, Collybistin Capture Antibody (unconjugated)\u003cbr\u003e\nDetector: ab317859, Collybistin Detector Antibody (unconjugated)\u003cbr\u003e\nThe reference range value is 0.16-10 ng\/mL.\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.\u003cbr\u003e\nOur carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg\/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with \u0026lt;1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nSandwich ELISA\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCollybistin also known as ARHGEF9 is a protein that functions as a guanine nucleotide exchange factor (GEF) primarily for the small GTPase Cdc42. This mechanism is essential in the regulation of actin cytoskeleton dynamics with an approximate molecular mass of 46 kDa. You can find Collybistin mainly in the brain where it plays a significant role in synaptic formation and function particularly in the postsynaptic sites of inhibitory synapses.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCollybistin plays a critical role in clustering and trafficking of specific proteins required for synapse maturation and stability. It participates as a part of a larger complex that includes key synaptic proteins like Gephyrin a scaffolding protein important for neurotransmitter receptor localization. This interaction between Collybistin and synaptic components ensures proper synaptic inhibitory signaling by controlling receptor clustering and distribution.\u003cbr\u003e\nPathways\u003cbr\u003e\nCollybistin is essential within the pathways associated with synaptic vesicle function and cytoskeletal dynamics. This protein interacts with important pathways like the Rho GTPase signaling pathway and Cdc42 effector pathways which are vital for cytoskeletal rearrangements. Through these pathways Collybistin engages with proteins such as F-actin influencing synaptic plasticity and neural connectivity by altering actin dynamics.\u003cbr\u003e\nCollybistin is linked to neurological conditions with particular relevance to epilepsy. Mutations in the Collybistin encoding gene ARHGEF9 have been associated with forms of epilepsy and intellectual disabilities. In these conditions disrupted interactions between Collybistin and other synaptic proteins like Gephyrin can lead to altered synaptic signaling illustrating the importance of its proper function in neurological health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851103686825,"sku":"ab317859","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab317859","provider":"Iright","version":"1.0","type":"link"}