{"product_id":"abcam-ab318137","title":"Abcam, ab318137, Anti-POLE (mutated P286R) antibody [EPR28640-56] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal POLE mutated PR antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), ICC\/IF, IHC-P, IP, Dot and reacts with Transfected cell lysate - Human, Synthetic peptide - Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR28640-56,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-P, Flow Cyt (Intra), Dot, IP, ICC\/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:We have not tested this antibody on endogenous material. We welcome any feedback from customers who have used this antibody in cell lines or tissue.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab318137 is the carrirer-free version of\u003cbr\u003e\nab318136\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe POLE protein also known as DNA polymerase epsilon catalytic subunit A functions as an important component in DNA replication and repair mechanisms. POLE possesses a molecular mass of approximately 261 kDa and demonstrates expression primarily in proliferating cells such as those in the bone marrow lymphoid tissues and the gastrointestinal tract. It operates within the eukaryotic DNA polymerase family distinct from other polymerases due to its high fidelity in DNA synthesis which minimizes errors during replication.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe POLE protein plays an essential role in the high-fidelity synthesis of leading-strand DNA during replication. POLE is part of a larger complex known as the DNA polymerase epsilon holoenzyme collaborating with accessory proteins that enhance its ability to synthesize DNA accurately. Moreover this protein contributes significantly to the proofreading function as it has intrinsic 3' to 5' exonuclease activity allowing the removal of incorrectly paired nucleotides therefore preventing mutations.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe POLE protein is fundamentally involved in the DNA replication and repair pathways. These pathways ensure genomic integrity and stability important for normal cell division and preventing genomic instability associated with cancer. POLE interacts with other proteins such as PCNA and RFC (replication factor C) within these pathways facilitating efficient replication fork progression and repair processes. POLE's activity ensures coordination with leading strand synthesis complementing the functions of other polymerases involved in lagging strand DNA synthesis.\u003cbr\u003e\nMutations in the POLE gene have been linked to colorectal cancer and endometrial cancer. These conditions are often characterized by a hypermutated phenotype arising from impaired DNA proofreading ability due to ineffective exonuclease activity. In this context POLE mutations can assume significance in sporadic cancer cases as well as in hereditary cancer syndromes where it interacts with mismatch repair proteins highlighting its role in maintaining genomic fidelity.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851880026281,"sku":"ab318137","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab318137","provider":"Iright","version":"1.0","type":"link"}