{"product_id":"abcam-ab318289","title":"Abcam, ab318289, Anti-AFM antibody [EPR25764-62] - BSA and Azide free (Capture)","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal AFM antibody. Carrier free. Suitable for sELISA and reacts with Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR25764-62,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab318289 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human AFM. The recommended pair for sandwich ELISA is:\u003cbr\u003e\nCapture: ab318289, Human AFM Capture Antibody (unconjugated)\u003cbr\u003e\nDetector:\u003cbr\u003e\nab318290\u003cbr\u003e\n, Human AFM Detector Antibody (unconjugated)\u003cbr\u003e\nThe reference range value is 15.63-2000 pg\/mL.\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.\u003cbr\u003e\nOur carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg\/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with \u0026lt;1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nSandwich ELISA\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAFM also known as Afamin is a glycoprotein assigned to the albumin family. Its molecular mass is approximately 87 kDa. This protein is mainly expressed in the liver and secreted into the bloodstream. It possesses binding affinity for vitamin E which suggests a role in transport and metabolic processes. It shares structural similarities with other proteins in the albumin family contributing to its functional versatility.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAFM plays a central role in the transport of lipids and vitamins particularly vitamin E throughout the body. It acts independently and does not participate in large protein complexes. AFM shows interactions with other lipid-binding proteins modulating their activities and ensuring efficient distribution of essential nutrients. Such interactions highlight the importance of AFM in maintaining cellular homeostasis and protecting cells from oxidative damage.\u003cbr\u003e\nPathways\u003cbr\u003e\nAFM actively participates in metabolic and vitamin transport pathways collaborating with proteins like albumin and transthyretin. It notably influences lipid metabolism and antioxidative pathways thereby contributing to overall cellular defense mechanisms. AFM's functionality intertwines with the activities of these proteins indicating its essential role in finely tuned biological processes especially those involving lipid and vitamin management.\u003cbr\u003e\nAFM has associations with metabolic and neurodegenerative conditions. Altered levels of AFM can correlate with diseases like type 2 diabetes and Alzheimer's disease. It interacts with other relevant proteins such as apolipoproteins impacting disease progression and symptomatology. These connections suggest that AFM could potentially be a biomarker or therapeutic target for managing such disorders opening avenues for future research and treatment strategies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851582132393,"sku":"ab318289","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab318289","provider":"Iright","version":"1.0","type":"link"}