{"product_id":"abcam-ab32042","title":"Abcam, ab32042, Anti-Cleaved Caspase-3 antibody [E83-77]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-Cleaved Caspase-3 antibody [E83-77] (ab32042) is a rabbit monoclonal antibody detecting Cleaved Caspase-3 in  Western Blot, ICC\/IF . Suitable for  Human, . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 610 publications - Trusted since 2006\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:E83-77,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:ICC\/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:This antibody is more sensitive for the detection of cleaved caspase-3 than for pro-caspase-3. For better observation of pro-caspase-3, we recommend ab32150.PubMedID 19789217 describes the detection of human cells injected into mice. The pro-caspase-3 is cleaved only when apoptosis event occurs. So, in order to detect active Caspase-3, we strongly suggest to induce your samples into apoptotic pathway.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAnti-Cleaved Caspase-3 antibody [E83-77] (ab32042) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC\/IF and WB.\u003cbr\u003e\nAnti-Cleaved Caspase-3 antibody [E83-77] (ab32042) was first used in a scientific publication in 1994 and has been cited over 610 times in peer reviewed journals. It's performance in Western Blot in human samplesis trusted by the scientific community.\u003cbr\u003e\nAbcam's high quality manufacturing and validation processes ensure Anti-Cleaved Caspase-3 antibody [E83-77] (ab32042) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.\u003cbr\u003e\nThe specificity of Anti-Cleaved Caspase-3 antibody [E83-77] (ab32042) has been confirmed by Western Blot testing in Cleaved Caspase-3 knockout HAP1 cells.\u003cbr\u003e\nAnti-Cleaved Caspase-3 antibody [E83-77] (ab32042) has 25 independent reviews from customers.\u003cbr\u003e\nAnti-Cleaved Caspase-3 antibody [E83-77] (ab32042) specifically detects Cleaved Caspase-3 (UniProt ID: P42574; Molecular weight: 17kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).\u003cbr\u003e\nConjugation-ready, carrier free format available for antibody clone E83-77 -\u003cbr\u003e\nab208003\u003cbr\u003e\nCleaved Caspase-3 is a crucial marker of apoptosis, the programmed cell death process. It is the activated form of Caspase-3, resulting from proteolytic cleavage. Cleaved Caspase-3 plays a vital role in executing apoptosis by cleaving various cellular substrates, leading to cell dismantling. Researchers use Cleaved Caspase-3 as a reliable indicator of apoptosis in studies of cancer, neurodegenerative diseases and other conditions involving cell death. Its detection is essential for understanding the mechanisms of apoptosis and developing targeted therapies.\u003cbr\u003e\nIn cancer research, cleaved Caspase-3 is used to assess the effectiveness of anti-cancer therapies that induce apoptosis in tumor cells. Elevated levels of cleaved Caspase-3 have been associated with better prognosis in certain cancers, such as oral tongue squamous cell carcinoma. Additionally, cleaved Caspase-3 has been implicated in non-apoptotic roles, such as promoting angiogenesis and chemotherapy resistance.\u003cbr\u003e\nNeurodegenerative Diseases: In neurodegenerative diseases like Alzheimer's (AD) and Parkinson's (PD) disease, cleaved Caspase-3 is involved in the apoptotic pathways that lead to neuronal cell death. For instance, caspase-3-cleaved Tau aggregates more easily than full-length Tau, contributing to the pathology of Alzheimer's disease.\u003cbr\u003e\nUnderstanding the role of cleaved Caspase-3 in these diseases helps researchers develop targeted therapies to mitigate neuronal loss and disease progression.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nSpecies reactivity\u003cbr\u003e\nMouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.\u003cbr\u003e\nPlease\u003cbr\u003e\ncontact us\u003cbr\u003e\nfor more information.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Cleaved Caspase-3 also known as active caspase 3 plays an important role in the execution phase of cell apoptosis. The caspase 3 molecule has a molecular weight of approximately 32 kDa. It undergoes cleavage into subunits that are important for its activation marking its conversion into the cleaved form. Cleaved caspase is widely expressed in various tissues with a notable presence in the cytoplasm of cells undergoing apoptotic processes. Cleavage of caspase 3 results in the formation of a functional enzyme that participates in the degradation of cellular components during apoptosis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCaspase 3 acts as an executioner enzyme in the process of programmed cell death. Its cleavage from the inactive zymogen form into the active form triggers apoptotic pathways ensuring the removal of damaged or unnecessary cells. Cleaved caspase operates within a proteolytic cascade often in conjunction with other caspases such as caspase 9. Once activated caspase 3 targets and cleaves specific cellular substrates contributing to the characteristic morphological changes and DNA fragmentation associated with apoptosis.\u003cbr\u003e\nPathways\u003cbr\u003e\nCleaved caspase 3 is significant within the intrinsic and extrinsic apoptotic pathways. It acts downstream of initiator caspases such as caspase 9 in the intrinsic pathway where its activation involves mitochondrial signals. In the extrinsic pathway it closely associates with caspase 8 mediating cell death signals from extracellular triggers. These pathways ensure that cleaved caspase 3 fulfills its role as a critical mediator of apoptosis linking upstream death signals to cellular dismantling during the apoptosis process.\u003cbr\u003e\nCleaved caspase 3 connects to conditions such as cancer and neurodegenerative diseases. In cancer dysregulation of caspase 3 activity may lead to evasion of apoptosis facilitating tumor progression. Therapeutic approaches aim to restore its apoptotic functions in cancer cells. In neurodegenerative diseases like Alzheimer's disease abnormal activation of cleaved caspase 3 contributes to neuronal loss. It interacts with proteins like amyloid precursor protein promoting the pathological changes associated with this disorder. Understanding these interactions provides insights into potential therapeutic avenues.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844030812329,"sku":"ab32042","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab32042","provider":"Iright","version":"1.0","type":"link"}