{"product_id":"abcam-ab32083","title":"Abcam, ab32083, Anti-SHP2 antibody [Y478]","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nAnti-SHP2 antibody [Y478] (ab32083) is a rabbit monoclonal antibody detecting SHP2 in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC\/IF . Suitable for  Human . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 10 publications - Trusted since 2006\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:Y478,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:ICC\/IF, WB, Flow Cyt (Intra), IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:This antibody recognises SHP2. This antibody is predicted to detect splice isoform 2 based on sequence analysis.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-SHP2 antibody [Y478] (ab32083) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human samples.\u003cbr\u003e\nWhat is the molecular weight of SHP2?\u003cbr\u003e\nAnti-SHP2 [Y478] (ab32083) specifically detects a band for SHP2 (UniProt: Q06124) at a molecular weight of 68kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-SHP2 [Y478] (ab32083) was first used in a scientific publication in 2006 and has been cited over 10 times in peer-reviewed journals.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nSpecificity confirmed\u003cbr\u003e\nThe specificity of Anti-SHP2 antibody [Y478] (ab32083) has been confirmed by Western blot testing in PTPN11 Knockout HAP1 cells.\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [Y478] also available for your convenience: ab32083, Carrier free -\u003cbr\u003e\nab182179\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab210616\u003cbr\u003e\n, PE -\u003cbr\u003e\nab305844\u003cbr\u003e\n, APC -\u003cbr\u003e\nab305845\u003cbr\u003e\n, HRP -\u003cbr\u003e\nab305846\u003cbr\u003e\n, Alkaline Phosphatase -\u003cbr\u003e\nab308735\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab309693\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab310059\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab311979\u003cbr\u003e\n, Alexa Fluor® 568 -\u003cbr\u003e\nab312453\u003cbr\u003e\n, Alexa Fluor® 750 -\u003cbr\u003e\nab321568\u003cbr\u003e\nSpecies reactivity\u003cbr\u003e\nMouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.\u003cbr\u003e\nPlease\u003cbr\u003e\ncontact us\u003cbr\u003e\nfor more information.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSHP2 also known as PTPN11 is a protein tyrosine phosphatase with a molecular mass of approximately 68 kDa. It is expressed in various tissues including the heart liver and immune cells. SHP2 belongs to the non-receptor class of protein tyrosine phosphatases and plays a critical role in cell signaling by acting as a regulator of signal transduction processes. SHP2 mediates these processes by dephosphorylating specific phosphotyrosine residues on target proteins influencing various cellular functions like proliferation differentiation and survival.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe role of SHP2 extends to involvement in several signaling cascades such as the Ras\/MAPK and PI3K\/AKT pathways. It functions as an essential component within protein complexes that facilitate cell communication and response to external signals. The protein modulates growth factor signaling and cytokine signaling highlighting its significance in normal cell function and development. SHP2's statement in signaling processes makes it an important regulator of cellular dynamics.\u003cbr\u003e\nPathways\u003cbr\u003e\nSHP2 participates in the Ras\/MAPK and PI3K\/AKT signaling pathways which are important for regulating cell growth survival and differentiation. Within these pathways SHP2 interacts with various signaling molecules including Grb2 Sos and Gab family adaptors. These interactions coordinate cellular responses to growth factors and other extracellular cues ensuring proper pathway activation and control. By serving as a critical mediator SHP2 integrates signals that are necessary for appropriate cellular outcomes.\u003cbr\u003e\nSHP2 is associated with several conditions such as Noonan syndrome and various cancers. Mutations in the PTPN11 gene which encodes SHP2 often result in aberrant signaling that leads to developmental anomalies or tumorigenesis. In Noonan syndrome the mutated SHP2 protein results in disrupted Ras\/MAPK pathway signaling. As for cancers SHP2 is often found to be overactive leading to enhanced cell proliferation and survival. In these contexts SHP2 is interconnected with other proteins like RAS and RAF which also contribute to oncogenic pathway activation and disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844108308649,"sku":"ab32083","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab32083","provider":"Iright","version":"1.0","type":"link"}