{"product_id":"abcam-ab32096","title":"Abcam, ab32096, Anti-CREB (phospho S133) antibody [E113]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-CREB (phospho S133) antibody [E113] (ab32096) is a rabbit monoclonal antibody detecting CREB in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC\/IF, Dot Blot . Suitable for  Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 230 publications - Trusted since 2006\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:E113,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:WB, IHC-P, IP, ICC\/IF, Dot, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:This antibody is specific for CREB phosphorylated on Serine 133. The immunogen of the antibody shares 94% homology with CREB (S136) and 86% homology with ATF1 (pS63). No experiment has been performed to verify the possible cross-reactivity.This antibody can't detect signal in mouse and rat brain related tissues in western blot.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nProduct Specifications\u003cbr\u003e\nAnti-CREB (phospho S133) antibody [E113] (ab32096) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC\/IF, IHC-P, IP, WB in human, mouse, rat samples.\u003cbr\u003e\nAnti-CREB (phospho S133) antibody [E113] (ab32096) specifically detects CREB Phospho-S133 (UniProt ID: P16220; Molecular weight: 35kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).\u003cbr\u003e\nQuality and Validation\u003cbr\u003e\nAbcam's high quality manufacturing and validation processes ensure Anti-CREB (phospho S133) antibody [E113] (ab32096) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.\u003cbr\u003e\nAnti-CREB (phospho S133) antibody [E113] (ab32096) has been cited over 230 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAnti-CREB (phospho S133) antibody [E113] (ab32096) has 13 independent reviews from customers.\u003cbr\u003e\nRelated Products\u003cbr\u003e\nConjugation-ready, carrier free format available for antibody clone E113 -\u003cbr\u003e\nab220798\u003cbr\u003e\nAntibody clone E113 is also available pre-conjugated to a variety of labels for your convenience - HRP (\u003cbr\u003e\nab219741\u003cbr\u003e\nTarget Information\u003cbr\u003e\nCREB (phospho S133) is a transcription factor that plays a vital role in regulating gene expression upon phosphorylation at serine 133. This phosphorylation enhances CREB's ability to activate genes involved in cell survival, metabolism, and proliferation. Dysregulation of CREB phosphorylation at S133 is implicated in several conditions, including cancer, where it aids in tumor development and resistance to apoptosis, and neurodegenerative disorders such as Alzheimer's disease, where it influences neuronal resilience and cognitive function.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Stable for 12 months at -20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCAMP response element-binding protein better known as CREB functions as a transcription factor that regulates gene expression. CREB protein operates by binding to specific DNA sequences called cAMP response elements in the promoters of target genes leading to increased or altered transcription. The protein has a molecular weight around 43 kDa. Expressed broadly in many tissues CREB's activity is particularly high in the brain where it plays key roles in neuronal functions. Researchers also refer to phospho-CREB when discussing the activated form of this protein often analyzed through methods like CREB Western blot.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCREB contributes significantly to cellular processes such as proliferation differentiation and survival through transcriptional regulation. CREB does not function alone; it frequently associates with other proteins forming complexes to exert its regulatory roles. Additionally CREB influences the development of memory and learning in neurons highlighting its extensive involvement in neurophysiological processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nCREB plays a substantial role in the cAMP signaling pathway as well as the mitogen-activated protein kinase (MAPK) pathway. Through its interaction with kinases and other signaling proteins CREB influences numerous downstream targets affecting cellular responses to external stimuli. Notably protein kinase A (PKA) activates CREB by phosphorylation facilitating its role in various signal transduction pathways and linking it to downstream transcriptional events.\u003cbr\u003e\nResearch links CREB to neurological disorders and some forms of cancer. Aberrant CREB activity associates with neurodegenerative diseases like Alzheimer’s where dysregulated gene expression impacts neuronal viability and function. Furthermore in cancer CREB's role in cell proliferation and survival ties it to oncogenes such as BCL2 where abnormal CREB function can promote tumorigenesis. Understanding these connections provides valuable insights for therapeutic strategies against such diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844053291177,"sku":"ab32096","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab32096","provider":"Iright","version":"1.0","type":"link"}