{"product_id":"abcam-ab32136","title":"Abcam, ab32136, Anti-Amyloid Precursor Protein antibody [Y188]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-Amyloid Precursor Protein antibody [Y188] (ab32136) is a rabbit monoclonal antibody detecting Amyloid Precursor Protein in  Western Blot, IP, IHC-P, ICC\/IF . Suitable for  Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 240 publications - Trusted since 2006\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:Y188,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:IP, WB, IHC-P, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:The antibody detects the C-terminal fragment of APP (APP-CTF).The immunogen used for this product is within Human Amyloid Precursor Protein aa 750 to the C-terminus and therefore may detect gamma secretase fragments 50, 57 and 59 in addition to fragments C31, C80, C83 and C99. Cross-reactivity with these fragments has not been confirmed experimentally.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAnti-Amyloid Precursor Protein antibody [Y188] (ab32136) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC\/IF, IHC-P, IP and WB.\u003cbr\u003e\nAnti-Amyloid Precursor Protein antibody [Y188] (ab32136) was first used in a scientific publication in 1984 and has been cited over 242 times in peer reviewed journals. It's performance in Western Blot in human, mouse and rat samples is trusted by the scientific community.\u003cbr\u003e\nAbcam's high quality manufacturing and validation processes ensure Anti-Amyloid Precursor Protein antibody [Y188] (ab32136) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.\u003cbr\u003e\nThe specificity of Anti-Amyloid Precursor Protein antibody [Y188] (ab32136) has been confirmed by Western Blot testing in Amyloid Precursor Protein knockout HEK-293T cells.\u003cbr\u003e\nAnti-Amyloid Precursor Protein antibody [Y188] (ab32136) has 8 independent reviews from customers.\u003cbr\u003e\nAnti-Amyloid Precursor Protein antibody [Y188] (ab32136) specifically detects Amyloid Precursor Protein (UniProt ID: P05067; Molecular weight: 86kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).\u003cbr\u003e\nConjugation-ready, carrier free format available for antibody clone Y188 -\u003cbr\u003e\nab220793\u003cbr\u003e\nAntibody clone Y188 is also available pre-conjugated to a variety of labels for your convenience - HRP, PE, Alexa Fluor\u003cbr\u003e\n488, Alexa Fluor\u003cbr\u003e\n647, Alexa Fluor\u003cbr\u003e\n594, Alexa Fluor\u003cbr\u003e\n568, Alexa Fluor\u003cbr\u003e\n555, Alexa Fluor\u003cbr\u003e\nab199550\u003cbr\u003e\nab208744\u003cbr\u003e\nab310994\u003cbr\u003e\nab311119\u003cbr\u003e\nab311748\u003cbr\u003e\nab313028\u003cbr\u003e\nab313229\u003cbr\u003e\nab321579\u003cbr\u003e\nAPP (Amyloid Precursor Protein) is a critical protein in neuro research, particularly for its role in the pathogenesis of Alzheimer's disease (AD). It is cleaved to produce amyloid-beta (beta-Amyloid, A-beta) peptides, which aggregate to form plaques, a hallmark of AD pathology. Researchers use APP to study the mechanisms of amyloid-beta production, aggregation and its effects on neuronal function and survival, making it essential for understanding and developing treatments for AD. Its expression and processing are key factors in the progression of neurodegenerative conditions.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Amyloid Precursor Protein (APP) also known as amyloid protein is a transmembrane protein that is approximately 695 to 770 amino acids in length depending on the isoform. The molecular mass of APP can vary but typically falls around 100 to 140 kDa. It is heavily expressed in the central nervous system particularly in neurons but also in other tissues like muscle and kidney. The APP undergoes proteolytic processing which leads to the generation of various fragments including beta-amyloid peptides.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe processing of APP plays a fundamental role in neuronal growth survival and repair. APP is cleaved into fragments that can regulate synaptic function and plasticity. It does not operate as a part of a complex but interacts with various cellular components. The protein participates in signaling pathways influencing cellular adhesion motility and neurite outgrowth. APP’s numerous interaction partners facilitate its involvement in different cellular processes highlighting its critical role in normal cell function.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe APP is a central component in the amyloidogenic pathway where its cleavage by beta-secretase and gamma-secretase yields beta-amyloid. This pathway is one of two primary metabolic routes for APP—alternative enzymatic processing through the non-amyloidogenic pathway precludes beta-amyloid formation releasing peptides that do not aggregate. Enzymes like BACE1 (beta-secretase 1) and presenilin are important in the amyloidogenic pathway directly resulting in the production of the neurotoxic amyloid beta-peptide.\u003cbr\u003e\nAPP is intensely linked to Alzheimer's disease and cerebral amyloid angiopathy. Accumulation of beta-amyloid peptides formed from APP cleavage is a hallmark of Alzheimer's disease leading to plaque formation in the brain. This aggregation impacts neuronal function and is associated with neurodegenerative processes. Interactions with proteins like tau are significant as tau also plays an essential role in Alzheimer's disease pathology. Misprocessing of APP and the resulting beta-amyloid aggregates are also contributors to cerebral amyloid angiopathy where deposits within cerebrovascular walls compromise vascular integrity.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844036284585,"sku":"ab32136","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab32136","provider":"Iright","version":"1.0","type":"link"}