{"product_id":"abcam-ab32143","title":"Abcam, ab32143, Anti-STAT3 (phospho S727) antibody [E121-31]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-STAT3 (phospho S727) antibody [E121-31] (ab32143) is a rabbit monoclonal antibody detecting STAT3 in  Western Blot, IP, IHC-P, ICC\/IF, Dot Blot . Suitable for  Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 80 publications - Trusted since 2006\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:E121-31,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:ChIC\/CUT\u0026amp;RUN-seq, WB, ICC\/IF, IHC-P, IP, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-STAT3 (phospho S727) antibody [E121-31] (ab32143) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF), Dot Blot in Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of STAT3?\u003cbr\u003e\nAnti-STAT3 (phospho S727) [E121-31] (ab32143) specifically detects a band for STAT3 (UniProt: P40763) at a molecular weight of 88kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-STAT3 (phospho S727) [E121-31] (ab32143) was first used in a scientific publication in 2006 and has been cited over 80 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-STAT3 (phospho S727) [E121-31] (ab32143) has over 5 independent reviews from customers.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [E121-31] also available for your convenience: ab32143, Carrier free -\u003cbr\u003e\nab219593\u003cbr\u003e\n, APC -\u003cbr\u003e\nab310853\u003cbr\u003e\n, PE -\u003cbr\u003e\nab310922\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab310947\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab311065\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab311650\u003cbr\u003e\n, Alexa Fluor® 568 -\u003cbr\u003e\nab312923\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab313135\u003cbr\u003e\n, Alexa Fluor® 750 -\u003cbr\u003e\nab321582\u003cbr\u003e\nSpecies reactivity\u003cbr\u003e\nMouse: We have preliminary internal testing data to indicate this antibody may not react with this species.\u003cbr\u003e\nPlease\u003cbr\u003e\ncontact us\u003cbr\u003e\nfor more information.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSignal Transducer and Activator of Transcription 3 (STAT3) also called p-stat3 or phospho-STAT3 is a critical transcription factor involved in various cellular processes. The molecular weight of STAT3 is approximately 92 kDa. This protein is expressed broadly across many tissues and cell types. Mechanically upon cytokine or growth factor stimulation STAT3 undergoes phosphorylation resulting in dimerization. This phosphorylated form often referred to as phospho-Stat3 or p-STAT3 translocates to the nucleus where it binds specific DNA sequences to modulate gene transcription.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSTAT3 is an important player in regulating cell growth and apoptosis. It functions not only as a transcription factor but also as part of a larger protein complex that operates within the cell nucleus to influence gene expression. This multifaceted role enables STAT3 to control the expression of genes related to cell proliferation survival and differentiation impacting various biological processes. These functions highlight its importance in tissue homeostasis and response to extracellular signals.\u003cbr\u003e\nPathways\u003cbr\u003e\nSTAT3 is actively involved in the JAK-STAT signaling pathway a principal route for many cytokines and growth factors and the MAPK pathway. STAT3 interacts with proteins such as JAK kinases which phosphorylate Stat3 and initiate the STAT3 signaling cascade. Additionally it closely associates with the protein Raf1 in the MAPK pathway linking external signals to transcriptional responses. These pathways play a significant role in immune response inflammation and growth signaling.\u003cbr\u003e\nResearchers have implicated STAT3 in cancer and autoimmune diseases. Persistent activation of STAT3 is often observed in various cancers including breast and lung cancer promoting oncogenesis through altered gene expression. Additionally abnormal STAT3 signaling is associated with autoimmune conditions such as rheumatoid arthritis. The interplay between STAT3 and proteins like IL-6 in these diseases highlights its potential as a therapeutic target for intervention.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844040872105,"sku":"ab32143","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab32143","provider":"Iright","version":"1.0","type":"link"}