{"product_id":"abcam-ab32150","title":"Abcam, ab32150, Anti-pro Caspase-3 antibody [E61]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nRabbit Recombinant Monoclonal Caspase-3 antibody. Suitable for IHC-P, IP, WB, ICC\/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 183 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:E61,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-P, Flow Cyt (Intra), ICC\/IF, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:The antibody only recognizes the pro-form of Caspase-3. It does not react with the cleaved forms (active enzyme) of Caspase-3.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nSpecies reactivity\u003cbr\u003e\nRat: We have preliminary internal testing data to indicate this antibody may not react with this species.\u003cbr\u003e\nPlease\u003cbr\u003e\ncontact us\u003cbr\u003e\nfor more information.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPro Caspase-3 also known as Caspase-3 zymogen is a central component in the apoptotic process. This inactive precursor form undergoes cleavage during apoptosis to become active caspase-3 an important executioner in cell death. Caspase-3 possesses a molecular weight of approximately 32 kDa. It is expressed ubiquitously in cells throughout the body with a higher concentration in tissues with rapid cell turnover such as thymus and gastrointestinal tract.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe inactive pro Caspase-3 form serves as a safeguard in cells preventing premature apoptosis. It only becomes the active caspase-3 after specific activation ensuring controlled progression of apoptosis. This inactive form does not participate in any complex but becomes a part of the caspase family network upon cleavage. In its active form caspase-3 leads to the cleavage of several key cellular proteins driving the morphological and biochemical changes seen during apoptosis.\u003cbr\u003e\nPathways\u003cbr\u003e\nPro Caspase-3 is critical in the apoptotic signaling pathway. It serves as a link between intrinsic and extrinsic pathways of apoptosis. The intrinsic pathway often triggered by mitochondrial signals and the extrinsic pathway initiated by death receptor signals both lead to the activation of caspase-3. In these pathways pro Caspase-3 interacts with proteins like caspase-8 and cytochrome c playing a part in their signaling cascades and cellular outcomes.\u003cbr\u003e\nPro Caspase-3 is associated with conditions involving dysregulated apoptosis. One such condition is cancer where evasion of apoptosis leads to uncontrolled cell proliferation. The therapeutic targeting of pro Caspase-3 activation holds potential in reinstating apoptotic pathways in cancerous cells. Additionally it relates to neurodegenerative diseases like Alzheimer's where excessive apoptosis contributes to neuronal loss. Here active caspase-3 interacts with proteins like amyloid precursor protein suggesting a role in the disease's progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844032417961,"sku":"ab32150","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab32150","provider":"Iright","version":"1.0","type":"link"}